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  1. 2014 iGEM Project
  2. 2017 experiments
  3. Alternative fats
  4. Alternative sugars
  5. Attempt SDS Page and also mini prep to confirm that yeast transformations worked
  6. Bacterial cultures
  7. Bureaucracy
  8. Bylaws
  9. CR 062214 humanKappaCasein 001
  10. CR 082514 humKappaCasein(Kex+)
  11. Checked for colony growth in 03Nov2014 streak plates. Result: Yes
  12. Checking for contamination in yeast strains, media, water, and lab surfaces 06Oct2014
  13. Cheese and Migranes
  14. Cheese cave
  15. Cheese making
  16. Cheese making/Experiment 1
  17. Cheese making/Experiment 2
  18. Cheese making/Experiment 3
  19. Chymosin
  20. Cloning 7 casein gBlocks, 12Sep2014 and on
  21. Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014
  22. Cloning human alpha casein S1, 04Oct2014 and on
  23. Cloning strategy
  24. DNA Handling
  25. Educational Videos
  26. Electra cloning of
  27. Ethical, Legal, and Social Implications
  28. Existing vegan cheeses
  29. Experiment to determine whether yeast is contaminated and to salvage strain using 5FOA, 23Oct2014
  30. Experiments started January 2016
  31. External communications
  32. Finances
  33. GBlock Design for Casein and FAM20C Kinase Genes
  34. GBlock design
  35. Growth Curve Experiment
  36. Health effects: allergy and cancer link
  37. Here
  38. How to take notes and record data
  39. IGEM Attributions
  40. IGEM Judging Feedback
  41. IGEM Modeling
  42. IGEM Official Team Profile
  43. IGEM Parts
  44. IGEM Poster & Presentation
  45. IGEM Project
  46. IGEM Team
  47. IGEM cloning team meeting notes 8/05/14
  48. IGEM team meeting nodes 6/16/14
  49. IGEM team meeting nodes 6/18/14
  50. IGEM team meeting nodes 6/2/14
  51. IGEM team meeting nodes 6/23/14
  52. IGEM team meeting nodes 6/25/14
  53. IGEM team meeting nodes 6/30/14
  54. IGEM team meeting nodes 6/9/14
  55. IGEM team meeting nodes 7/14/14
  56. IGEM team meeting nodes 7/20/14
  57. IGEM team meeting nodes 7/28/14
  58. IGEM team meeting nodes 7/7/14
  59. IGEM team meeting nodes 8/11/14
  60. IGEM team meeting nodes 8/18/14
  61. IGEM team meeting nodes 8/25/14
  62. IGEM team meeting nodes 8/4/14
  63. IGEM team meeting nodes 9/1/14
  64. IGEM team meeting nodes 9/15/14
  65. IGEM team meeting nodes 9/29/14
  66. IGEM team meeting nodes 9/8/14
  67. IGEM team meeting notes 1/5/15
  68. IGEM team meeting notes 10/06/14
  69. IGEM team meeting notes 10/13/14
  70. IGEM team meeting notes 10/20/14
  71. IGEM team meeting notes 10/27/14
  72. IGEM team meeting notes 11/10/14
  73. IGEM team meeting notes 11/17/14
  74. IGEM team meeting notes 11/24/14
  75. IGEM team meeting notes 11/3/14
  76. IGEM team meeting notes 12/1/14
  77. IGEM team meeting notes 12/15/14
  78. IGEM team meeting notes 12/22/14
  79. IGEM team meeting notes 12/8/14
  80. IGEM team meeting notes 3/24/14
  81. IGEM team meeting notes 3/31/14
  82. IGEM team meeting notes 4/21/14
  83. IGEM team meeting notes 4/28/14
  84. IGEM team meeting notes 5/06/14
  85. IGEM team meeting notes 5/12/14
  86. IGEM team meeting notes 5/19/14
  87. IGEM team meeting notes 5/26/14
  88. IGEM team meeting notes 6/16/14
  89. IGEM team meeting notes 6/18/14
  90. IGEM team meeting notes 6/2/14
  91. IGEM team meeting notes 6/23/14
  92. IGEM team meeting notes 6/25/14
  93. IGEM team meeting notes 6/30/14
  94. IGEM team meeting notes 6/9/14
  95. IGEM team meeting notes 7/14/14
  96. IGEM team meeting notes 7/20/14
  97. IGEM team meeting notes 7/28/14
  98. IGEM team meeting notes 7/7/14
  99. IGEM team meeting notes 8/11/14
  100. IGEM team meeting notes 8/18/14
  101. IGEM team meeting notes 8/25/14
  102. IGEM team meeting notes 8/4/14
  103. IGEM team meeting notes 9/1/14
  104. IGEM team meeting notes 9/15/14
  105. IGEM team meeting notes 9/29/14
  106. IGEM team meeting notes 9/8/14
  107. IndieGogo RVC Page
  108. Initial studies of yeast strains' growth conditions and troubleshooting
  109. Introductory textbook
  110. January2016/Experimental design
  111. January2016/Plasmid design
  112. Life Cycle Assessments and Techno-Economic Analyses
  113. Link
  114. Live streaming
  115. Mailing lists
  116. Making liquid cultures, in duplicate, of bovine Alpha S1 and bovine Beta B transformed yeast colonies.
  117. Media
  118. Microbiology
  119. Microwaving agar
  120. Molecular Experiments
  121. Molecular biology
  122. Narwhal Cheese
  123. New 5 FOA Plates and prepare media
  124. Notebook
  125. Phosphorylation and glycosylation of caseins
  126. Plasmid design
  127. Plating URA- and Turbo Yeast Control
  128. Plating URA- and turbo Yeast Control
  129. Presenting
  130. Product viability
  131. Protease problems
  132. Protein purification
  133. Quantified IDT DNA (human kappa casein kex-) sample on nanodrop spectrophotometer to verify that the resuspension back in September worked.
  134. Quantified IDT DNA (human kappa casein kex-) sample on nanodrop spectrophotometer to verify that the resuspension back in September worked. Checked for colony growth in 03Nov2014 streak plates.
  135. Questions and Answers
  136. Raw Experimental Notes June 2016
  137. Real Vegan Cheese
  138. Real Vegan Cheese Notebook Documentation Guidelines
  139. Real Vegan Cheese Notebook Training Sign-off
  140. References on casein electrophoresis
  141. Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2
  142. Repeat Mini Prep of bovine alpha S1 and bone beta B yeast
  143. Repeat SDS Page-Gel for all the bovine proteins
  144. SDS Page-Gel for all the bovine casein inserts
  145. SDS Page-Gel for all the bovine proteins
  146. SDS Page-Gel for all the bovine proteins using micellar casein from Nutribio
  147. Safety
  148. Science
  149. Sequencing Analysis using Serial Cloner 2-6-1
  150. Service and reagent providers
  151. Shared Laboratory Notebook
  152. Similar projects
  153. Software
  154. Streaked new colonies of Bovine alpha S1 and Bovine beta B casein yeast transformations 03Nov2014
  155. Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014
  156. Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing 10Oct2014
  157. Submitting FAM20C (Kex + & Kex -) for sequencing with new internal primers 14Oct2014
  158. Test yeast transformation Bovine alpha S1 and Bovine beta B caseins 26Oct2014
  159. Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S
  160. Updates
  161. Vegan growth media
  162. Video
  163. Video/Purchase list
  164. Video conferencing
  165. Video recording
  166. Western Blot of bovine casein
  167. Working Groups
  168. Yeast Transformation
  169. Yeast Transformation (Clontech kit)
  170. Yeast Transformation of All Bovine Casein Inserts

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