IGEM team meeting nodes 7/20/14
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* Call-in info:
* \url{https://zoom.us/j/820128495} Or, go to \url{https://zoom.us/join} and enter meeting ID: 820 128 495
Note: Alisa Cordesius from IndieGogo may give us a call with suggestions for how to improve our campaign!
##I. Intro/Go around
* Meeting Attendees: (zoom): Advait , Maria, Wes
* CCL: Patrik, Lou, Beth, Rachel, Ryan, Jamie, Ahnon
*
##II. Report back to-does from last meeting
* TO-DO list from last week:
*
* 1. Yeast Strain - finish registration on ATCC (Credit refs)
* 2. Next meeting at BioCurious: have a planned experiment ready for the meeting; contact Nicola to coordinate details
* 3. Contact sequentech about sequencing reactions and primer design -
* 4. Modify experiment timeline google doc: add additional sheets to overall document, one for each month, to break monthly calendar schedule into a week-by-week schedule for precise tuning of progress
*
##III. IndieGogo progress
* Urgent: Anybody for a **underlinelive Radio Interview tomorrow morningunderline**? \url{https://groups.google.com/forum/#}!topic/ccl-igem/h0b2vIT6Riw
* Jamie will do the interview
* Help optimize our content! Lots of good suggestions from Antony \& others - need more people to help implement.
* What is our *next* stretch goal. Should have a graphic showing our funding horizon doesn't end at $20K
* Next update: what? Could do 3D printed prototypes
* Contact remaining "Tell Us Why We're Wrong" and "Ask a Biohacker" donors. (see \url{https://pad.riseup.net/p/socialcheesemedia} )
* Write new article to submit to other magazines/newspapers/news stations **or get more people to write articles for us**
* \url{https://docs.google.com/document/d/1UsZk7yiUDEB118iMZ22Fg3j2nRMyBxvLEmku\_bfl25k/edit}
IIIa Stretch goals
* Mammoth Cheese - problem - unecc glamourous, not taken seriously
* Bioreactor - 1 large - need might not be understood by general public, but sounds cool
* Bioreactor - several small
* Micelle equipment: particle analyzer
* Endangered - black-footed ferret - too glam but may raise awareness of endangered species... but might be too much new info which muddies/decreases understanding of both projects
Media Contacts
* Wired - we have several contacts
* KQED - Patrik talked to her
Why cheese instead of Milk?
* Cheese is harder (micelle formation, etc.)
* Milk is being done by Muufri
##IV. Complete the new safety form for igem together
\url{http://igem.org/Safety/Safety\_Form?team\_id=1531}
* - Who has taken the Biocurious Safety Training and who hasn't?
* Has:
* Has not:
* Or, complete the safety training together (DO NOT USE QUIZ LINK YET!)
*
##V. Work on the experiment plan/schedule
* -\url{https://docs.google.com/spreadsheets/d/1NOPblWr9\_pIHMst0Nvy\_\_7J\_n97LNwEsz2ujtZ3yFBo/edit#gid=0}
* -\url{https://www.google.com/calendar/render?pli=1}
== Molecular Biology Experiments ==
Run SDS-PAGE gels on K-casein from E. coli
Order primers to verify construct
Send out K-casein for sequencing
Make competent yeast
Transform yeast
Run SDS-PAGE gels on K-casein from yeast
Things we need:
- Uracil
- Reagents to induce competence
- Buy gels or pour our own? BUY! We gots $
- SDSbuffer, loading dye and Molecular Weight marker.
- Primers
-
== Cheese experiment 2 ==
Tenatively scheduled for the Omni 4799 Shattuck Ave July 28 7PM
Can we dissolve casein powder, and make cheese out of it?
Stuff to bring:
*Matts high-shear blender
*jewelry cleaning sonicator (patrik will bring)
*Do we have lab-sonicators? (may be more adjustable)
* heater (juul will verify if omni has stoves)
* pH meter (juul will order one, lou will check home depot and aquariam store)
* chymosin (juul will bring)
* white vinegar (lou will bring)
* thermometer (digital ?) (juul \& lou will bring)
* cheese cloth (Patrik will bring)
* pot (lou will bring)
* CaCl (juul will bring)
* test tubes / glasswares
* ice (lou will bring)
David Poole agreed to bring the following.
1- micellar casein powder (oddly missing from our list)
2- whole milk (control)
3- fat free milk
4- milk-free fat
5- citric acid
6- extra calcium salt
7- extra rennin/chymosin
8- extra jewelry sonicator
9- papers on prior work from the journal of dairy science
10- additional stuff I've found useful for making cheese (e.g. a salad spinner, thermometer etc)
Parameters to vary: pH, Ca, T, sonication/shear
test using chymosin, and settling over time
ToDo:
*Collect all papers relevant to experiment, and give access to everyone (juul)
*Solicit questions for ask a biohacker and tell us why we're wrong (juul)
*Research protein purification methods (juul)
*Organize / set up inventory