IGEM team meeting notes 10/06/14

Meeting 10/06/2014 at BioC

Attendees:

   Call-in info: https://zoom.us/j/820128495 Or, go to https://zoom.us/join and enter meeting ID: 820 128 495  

   Biocurious: Johan, Advait, Maria, Shashank, Patrik, Nikola, Emi, Stephanie, Peter, Romie, Anja, Lafia, Santos
   Zoom: Rebecca, Moises, Craig
   Pad: Juul
   

Annoucenements:

• BioBrick DNA is dues at iGEM by October 10!
• Live streaming setup is opearational. Still some more work to do.

TODO this week:

• ...

Troubleshooting failed experiment

   -Get fresh dna 2.0 reagents, and then retry cloning?

   -Shashank: Retry tonight w/ heat shock?

   -Craig: order new cells, and new electra cloning reagents

   *We need to keep track of keeping things at their right temperature.

   *Getting new dH5 alpha tomorrow

FINAL DECISION: -Transform 1 microliter into 10 microliters today -Transform 1 microliter into 10 microliters tomorrow -If both don't work, then order new electra reagents

• Get biobricks designed!!

   -Figure out how to order kit (NEB access), and other things to do for this 

   -Johan can order from NEB

   -Submission requirements: http://parts.igem.org/DNA_Submission

   -The Registry accepts miniprepped plasmid DNA (BioBrick RFC10 compatible part samples in pSB1C3, at least 10ul of 25ng/ul, or 250ng total if dried down) in single PCR tubes, 8-tube strips, or 96-well plates. Users must provide a tracking number for their shipment on the submission form, as this allows the Registry to keep track of shipments should there be customs or delivery issues.

Collobration with The Tech Museum. Romie (rlittrel@thetech.org) and (ascholze@thetech.org) came to our meeting to offer help and collobration on the project. They are also doing a community iGEM project and as part of it need to collobrate with other labs and they have offered to let us store things in their -80c freezer and to use their NEB iGEM kit. If anyone is available please email them and we can build the biobrick with them or just get the NEB from them and do it at bioc. Further opportunities for promoting the project and interacting with the public in the future. They will have an established relationship with BioC after the next BioC board meeting which Romie plans to attend.

keep adding TODO items here (preferably with names!) as they come up during the meeting...

   Poster design for jamboree

   Poster guidelines

   Be sure to look at the poster guidelines and judging requirements and keep them in mind as you begin creating your team poster! Information about the expected components, evaluation criteria, and the judging process can be found on the Poster Guidelines page.

   Design your team banner!

   This year you can design a team banner and we will print for you! 

   The banners will be displayed at the Giant Jamboree for everyone to see. Banner designs are due October 13, and more details are available on the Banner page.

Agenda

   Let's make sure *everybody* has access to the wiki!

   What's left to do for iGEM DNA submission?

   ...

Yeast URA- negative controls: Aims/Purpose: We're getting colony growth on *all* of our media, including apparent growth of our two URA- yeast strains on the CM uracil negative medium. Under the microscope, the cells look like yeast. *Something* must be getting contaminated, likely with WT S. cerevisiae from the lab, or environmental yeast strains. We should test all possible sources of contamination, including water sources, test for yeast on various surfaces, etc.

Controls:

   - open in air on bench, 1hr
   - open in air inside BSC, 1hr
   - 10ul of yeast droplets on plate
   - 10ul water droplets
   - swab all surfaces, and streak on plate in quarters

   - one plate for bench surfaces

   - one plate surfaces inside BSC

   - one plate for others: pipetters, gloves, pipette tips, 

   - on plate for handles: fridge, cabinets, drawers,