IGEM team meeting notes 7/7/14

Meeting 07/07/2014

Attendees: Patrik D., Maria I., Ben, Steven, Eric, Vern, Itai, Arif, Ed, Wes, Brett, Nokolai, Lou, Teddy, Cherie, Tito, Eri, Advait, Craig, via Zoom: Ahnon

Call in information: https://zoom.us/j/744239720 Or, go to https://zoom.us/join and enter meeting ID: 744 239 720

Ellen Jorgensen* will join us from 6:30-7:30pm. http://www.meetup.com/BioCurious/events/189694362/ 6:30-7pm: Ellen covers the Community Labs iGEM track. Tips, meaning, context and such 7-7:30pm: Ellen fields any questions we have about iGEM (meeting requirements, best ways to do outreach..really anything)

Indiegogo Update https://www.indiegogo.com/projects/real-vegan-cheese/

Budget planning. Who is the money umbrelled under for tax purposes so that no one has to individually deal with it on their personal taxes. Would it make sense to have a bank account in orginizations name and have it under one of two labs with its own 501c3.

  Committee???

Funnel money through Food not bombs? Members for Budget Committee/purchasing (how much do we have, how to spend it) : Patrik

No experiments without budget approval. Process for submitting experiments:

timeline https://docs.google.com/spreadsheets/d/1NOPblWr9_pIHMst0Nvy__7J_n97LNwEsz2ujtZ3yFBo/edit#gid=0

Prioritizing reagent purchases.

We need to finalize stretch goals (bioreactor; experimenting with pichia; iGEM travel expenses?) Send folks to iGEM. How many people want to go? Sending 5 scientists to iGem (travel plus hotel etc) Narwhal cheese - more exciting Pichia - ethichal issues movign away from bakers yeast? Equipment - investment into lab Bioreactor -

We also need to put up the bank information for receiving funds from indiegogo. It is currently Ashley's Paypal account, need to find a 501c3

Develop our next indiegogo campaign update?

Plan video responses to 'ask a biohacker'?

Experiment Planning - So much science, so little time!

A. Review current experiments B. Future experiments (like, in the next 3 weeks). 1) Sequencing hKCasein clones - 15-20 reactions free from Sequetech. - need to design sequencing primers

2) SDS-Page of supernatants from E. coli o/n cultures - did we see expression?

3) Transforming Yeast - Needs: Yeast (Ura3-), YPD agar +/- uracil and broth, transformation reagents

4) Cloning other constructs. - Place order with IDT?

5) "Making cheese" experiments - Dry casein resuspended in different solvents (fat, water, etc)

C. Planning next working meetings - when will we do these experiments? Need casein by August 1.

Ethics conversation: Our website needs to be updated with our commitment to 'biohacker' ethics, a GMO statement, et cetera. Is the ethics team working on this stuff??

TO DO:

Research Plan Cloning, verification, purification of proteins

Stretch Goals Sending folks to iGEM - what are requirements to go? Picchia pastoris Narwahl - Check in with UC Santa Cruz - follow up Bioreactor Other equipment

Update for IndieGoGo

Contact Food not Bombs for fiscal sponsorship

Ethical Team: Maria, Craig, Rebecca Biohacker ethics.

• Ellen Jorgensen is cofounder & ED at Genspace and cochair of the Community Labs track at iGEM. She's helped teams compete in iGEM for years through Genspace

Stretch goals brainstorm:

   Teddy: maybe focus on stretch goals that directly help the vegan cheese project. This way we can focus mainly on the Real Vegan Cheese project and have iGEM just serve as a great platform for publicity to further bolster the actual Vegan Cheese Project (e.g. narwhal cheese, bioreactor, etc.)