User contributions for Rachel Linzer
Jump to navigation
Jump to search
- 23:28, 30 September 2014 diff hist +160 N File:Samples details from Sequetech sequence pick-up, 30Sep2014.png This table contains sample name, ID, size, primer, DNA concentration and purity details for samples submitted to Sequetech for sequencing on September 30, 2014. current
- 23:26, 30 September 2014 diff hist +81 Shared Laboratory Notebook
- 11:14, 30 September 2014 diff hist +2,375 Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 →Cloning bovine beta casein and Fam20C Kex +/- gBlocks into pD1214, transforming into E. coli, extracting and sequencing plasmid DNA
- 10:20, 30 September 2014 diff hist +2,038 Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014
- 07:19, 30 September 2014 diff hist +122 Existing vegan cheeses →Opinions
- 07:13, 30 September 2014 diff hist +378 Existing vegan cheeses →Daiya
- 06:57, 30 September 2014 diff hist +39 Cloning strategy →Progress summary, milestones 1 & 2
- 03:59, 30 September 2014 diff hist +117 Cloning 7 casein gBlocks, 12Sep2014 and on →Plasmid DNA quantification & purity spec readings for 14Sep2014 midipreps
- 03:56, 30 September 2014 diff hist +19 Real Vegan Cheese →Project pages
- 03:56, 30 September 2014 diff hist −54 Shared Laboratory Notebook
- 03:55, 30 September 2014 diff hist +7,261 N GBlock design Created page with "=Codon Optimization, Restriction Analysis and Flanking Sequences for Casein and FAM20C Kinase Genes= ==Overview== In this section, we use published amino acid sequences for ou..."
- 02:34, 30 September 2014 diff hist 0 N File:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf current
- 19:22, 29 September 2014 diff hist +618 GBlock Design for Casein and FAM20C Kinase Genes →Disruption of Kex protease amino acid recognition sites current
- 19:03, 29 September 2014 diff hist +5,488 GBlock Design for Casein and FAM20C Kinase Genes →Overview
- 15:48, 29 September 2014 diff hist +28 m Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 →Cloning casein & FAM20C gBlocks into pD1214 & transforming plasmids into E. coli
- 08:57, 29 September 2014 diff hist −3 m GBlock Design for Casein and FAM20C Kinase Genes →Overview
- 08:52, 29 September 2014 diff hist +1,158 N GBlock Design for Casein and FAM20C Kinase Genes Created page with "=Codon Optimization, Restriction Analysis and Flanking Sequences for Casein and FAM20C Kinase Genes= ==Overview== In this section, we describe using published amino acid seque..."
- 08:10, 29 September 2014 diff hist 0 m Shared Laboratory Notebook
- 08:09, 29 September 2014 diff hist +54 Shared Laboratory Notebook
- 08:02, 29 September 2014 diff hist +22 m Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 →Cloning casein & FAM20C gBlocks into pD1214 & transforming plasmids into E. coli
- 08:00, 29 September 2014 diff hist +31 m Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 →Genes to be cloned & pD1214 vector
- 07:49, 29 September 2014 diff hist 0 Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S →Overview current
- 07:48, 29 September 2014 diff hist +5,568 N Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S Created page with "= Troubleshooting failed transformation of P.FAKS.humBeta.S:pD1214 into dH5alpha ''E. coli''= == Overview == Of the September 12, 2014 transformations (gBlocks:pD1214 to the ..."
- 07:34, 29 September 2014 diff hist −4,592 Cloning 7 casein gBlocks, 12Sep2014 and on
- 07:25, 29 September 2014 diff hist +73 Shared Laboratory Notebook
- 07:24, 29 September 2014 diff hist +18 m Cloning 7 casein gBlocks, 12Sep2014 and on →Overview
- 01:14, 29 September 2014 diff hist +256 Cloning 7 casein gBlocks, 12Sep2014 and on →Repeating Electra cloning and transformation for P.FAKS.humBeta.S
- 01:08, 29 September 2014 diff hist +33 m Cloning 7 casein gBlocks, 12Sep2014 and on →Repeating transformation of P.FAKS.humBeta.S:pD1214 into E. coli
- 00:44, 29 September 2014 diff hist +9 m Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 →Cloning casein gBlocks into pD1214 & transforming plasmids into E. coli
- 00:43, 29 September 2014 diff hist +1 m Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 →Cloning casein gBlocks into pD1214 & transforming plasmids into E. coli
- 00:41, 29 September 2014 diff hist −6 m Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 →Cloning casein gBlocks into pD1214 & transforming plasmids into E. coli
- 00:38, 29 September 2014 diff hist +2,761 Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 →Cloning casein gBlocks into pD1214 & transforming plasmids into E. coli
- 00:08, 29 September 2014 diff hist +2,221 N Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 Created page with "= Cloning bovine beta casein and Fam20C Kex +/- gBlocks into pD1214, transforming into ''E. coli'', extracting and sequencing plasmid DNA= == Overview == During this series ..."
- 23:39, 28 September 2014 diff hist +68 Shared Laboratory Notebook
- 23:34, 28 September 2014 diff hist −4 m Cloning 7 casein gBlocks, 12Sep2014 and on →Midiprep of P.FAKS.humBeta.S:pD1214 from dH5alpha E. coli
- 00:29, 28 September 2014 diff hist +462 Cloning 7 casein gBlocks, 12Sep2014 and on →Midiprep of P.FAKS.humBeta.S:pD1214 from dH5alpha E. coli
- 22:49, 27 September 2014 diff hist 0 m Cloning strategy →Progress summary, milestones 1 & 2
- 21:06, 27 September 2014 diff hist +279 Cloning 7 casein gBlocks, 12Sep2014 and on →Midiprep of P.FAKS.humBeta.S:pD1214 from dH5alpha E. coli
- 20:54, 27 September 2014 diff hist +92 Cloning 7 casein gBlocks, 12Sep2014 and on →Cloning 7 casein gBlocks into pD1214, transforming into E. coli, extracting and sequencing plasmid DNA
- 20:51, 27 September 2014 diff hist +96 Cloning 7 casein gBlocks, 12Sep2014 and on →Repeating Electra cloning and transformation for P.FAKS.humBeta.S
- 20:46, 27 September 2014 diff hist +1,374 Cloning 7 casein gBlocks, 12Sep2014 and on →Repeating Electra cloning and transformation for P.FAKS.humBeta.S
- 20:44, 27 September 2014 diff hist +116 Cloning 7 casein gBlocks, 12Sep2014 and on →Cloning casein gBlocks into pD1214 & transforming plasmids into E. coli
- 20:37, 27 September 2014 diff hist +138 Cloning 7 casein gBlocks, 12Sep2014 and on →Plasmid midipreps of successful 12Sep2014 transformations
- 20:35, 27 September 2014 diff hist +6 Cloning 7 casein gBlocks, 12Sep2014 and on →Plasmid midipreps of successful 12Sep2014 transformations
- 20:32, 27 September 2014 diff hist +166 CLONE 082514 humKappaCasein(Kex+) →Experiment 082514_humKappaCasein(Kex+) Cloning
- 20:28, 27 September 2014 diff hist +149 Cloning strategy →Progress summary, milestones 1 & 2
- 20:21, 27 September 2014 diff hist +1 CLONE 082514 humKappaCasein(Kex+) →Experiment 082514_humKappaCasein(Kex+) Cloning
- 20:20, 27 September 2014 diff hist +1,335 CLONE 082514 humKappaCasein(Kex+) →Experiment 082514_humKappaCasein(Kex+) Cloning
- 20:03, 27 September 2014 diff hist +8 m Cloning 7 casein gBlocks, 12Sep2014 and on →Cloning casein gBlocks into pD1214 & transforming plasmids into E. coli
- 19:59, 27 September 2014 diff hist +52 Cloning 7 casein gBlocks, 12Sep2014 and on →Repeating Electra cloning and transformation for P.FAKS.humBeta.S