User contributions for Rachel Linzer
Jump to navigation
Jump to search
- 23:28, 30 September 2014 diff hist +160 N File:Samples details from Sequetech sequence pick-up, 30Sep2014.png This table contains sample name, ID, size, primer, DNA concentration and purity details for samples submitted to Sequetech for sequencing on September 30, 2014. current
- 23:26, 30 September 2014 diff hist +81 Shared Laboratory Notebook
- 11:14, 30 September 2014 diff hist +2,375 Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 →Cloning bovine beta casein and Fam20C Kex +/- gBlocks into pD1214, transforming into E. coli, extracting and sequencing plasmid DNA
- 10:20, 30 September 2014 diff hist +2,038 Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014
- 07:19, 30 September 2014 diff hist +122 Existing vegan cheeses →Opinions
- 07:13, 30 September 2014 diff hist +378 Existing vegan cheeses →Daiya
- 06:57, 30 September 2014 diff hist +39 Cloning strategy →Progress summary, milestones 1 & 2
- 03:59, 30 September 2014 diff hist +117 Cloning 7 casein gBlocks, 12Sep2014 and on →Plasmid DNA quantification & purity spec readings for 14Sep2014 midipreps
- 03:56, 30 September 2014 diff hist +19 Real Vegan Cheese →Project pages
- 03:56, 30 September 2014 diff hist −54 Shared Laboratory Notebook
- 03:55, 30 September 2014 diff hist +7,261 N GBlock design Created page with "=Codon Optimization, Restriction Analysis and Flanking Sequences for Casein and FAM20C Kinase Genes= ==Overview== In this section, we use published amino acid sequences for ou..."
- 02:34, 30 September 2014 diff hist 0 N File:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf current
- 19:22, 29 September 2014 diff hist +618 GBlock Design for Casein and FAM20C Kinase Genes →Disruption of Kex protease amino acid recognition sites current
- 19:03, 29 September 2014 diff hist +5,488 GBlock Design for Casein and FAM20C Kinase Genes →Overview
- 15:48, 29 September 2014 diff hist +28 m Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 →Cloning casein & FAM20C gBlocks into pD1214 & transforming plasmids into E. coli
- 08:57, 29 September 2014 diff hist −3 m GBlock Design for Casein and FAM20C Kinase Genes →Overview
- 08:52, 29 September 2014 diff hist +1,158 N GBlock Design for Casein and FAM20C Kinase Genes Created page with "=Codon Optimization, Restriction Analysis and Flanking Sequences for Casein and FAM20C Kinase Genes= ==Overview== In this section, we describe using published amino acid seque..."
- 08:10, 29 September 2014 diff hist 0 m Shared Laboratory Notebook
- 08:09, 29 September 2014 diff hist +54 Shared Laboratory Notebook
- 08:02, 29 September 2014 diff hist +22 m Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 →Cloning casein & FAM20C gBlocks into pD1214 & transforming plasmids into E. coli
- 08:00, 29 September 2014 diff hist +31 m Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 →Genes to be cloned & pD1214 vector
- 07:49, 29 September 2014 diff hist 0 Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S →Overview current
- 07:48, 29 September 2014 diff hist +5,568 N Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S Created page with "= Troubleshooting failed transformation of P.FAKS.humBeta.S:pD1214 into dH5alpha ''E. coli''= == Overview == Of the September 12, 2014 transformations (gBlocks:pD1214 to the ..."
- 07:34, 29 September 2014 diff hist −4,592 Cloning 7 casein gBlocks, 12Sep2014 and on
- 07:25, 29 September 2014 diff hist +73 Shared Laboratory Notebook
- 07:24, 29 September 2014 diff hist +18 m Cloning 7 casein gBlocks, 12Sep2014 and on →Overview
- 01:14, 29 September 2014 diff hist +256 Cloning 7 casein gBlocks, 12Sep2014 and on →Repeating Electra cloning and transformation for P.FAKS.humBeta.S
- 01:08, 29 September 2014 diff hist +33 m Cloning 7 casein gBlocks, 12Sep2014 and on →Repeating transformation of P.FAKS.humBeta.S:pD1214 into E. coli
- 00:44, 29 September 2014 diff hist +9 m Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 →Cloning casein gBlocks into pD1214 & transforming plasmids into E. coli
- 00:43, 29 September 2014 diff hist +1 m Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 →Cloning casein gBlocks into pD1214 & transforming plasmids into E. coli
- 00:41, 29 September 2014 diff hist −6 m Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 →Cloning casein gBlocks into pD1214 & transforming plasmids into E. coli
- 00:38, 29 September 2014 diff hist +2,761 Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 →Cloning casein gBlocks into pD1214 & transforming plasmids into E. coli
- 00:08, 29 September 2014 diff hist +2,221 N Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 Created page with "= Cloning bovine beta casein and Fam20C Kex +/- gBlocks into pD1214, transforming into ''E. coli'', extracting and sequencing plasmid DNA= == Overview == During this series ..."
- 23:39, 28 September 2014 diff hist +68 Shared Laboratory Notebook
- 23:34, 28 September 2014 diff hist −4 m Cloning 7 casein gBlocks, 12Sep2014 and on →Midiprep of P.FAKS.humBeta.S:pD1214 from dH5alpha E. coli
- 00:29, 28 September 2014 diff hist +462 Cloning 7 casein gBlocks, 12Sep2014 and on →Midiprep of P.FAKS.humBeta.S:pD1214 from dH5alpha E. coli
- 22:49, 27 September 2014 diff hist 0 m Cloning strategy →Progress summary, milestones 1 & 2
- 21:06, 27 September 2014 diff hist +279 Cloning 7 casein gBlocks, 12Sep2014 and on →Midiprep of P.FAKS.humBeta.S:pD1214 from dH5alpha E. coli
- 20:54, 27 September 2014 diff hist +92 Cloning 7 casein gBlocks, 12Sep2014 and on →Cloning 7 casein gBlocks into pD1214, transforming into E. coli, extracting and sequencing plasmid DNA
- 20:51, 27 September 2014 diff hist +96 Cloning 7 casein gBlocks, 12Sep2014 and on →Repeating Electra cloning and transformation for P.FAKS.humBeta.S
- 20:46, 27 September 2014 diff hist +1,374 Cloning 7 casein gBlocks, 12Sep2014 and on →Repeating Electra cloning and transformation for P.FAKS.humBeta.S
- 20:44, 27 September 2014 diff hist +116 Cloning 7 casein gBlocks, 12Sep2014 and on →Cloning casein gBlocks into pD1214 & transforming plasmids into E. coli
- 20:37, 27 September 2014 diff hist +138 Cloning 7 casein gBlocks, 12Sep2014 and on →Plasmid midipreps of successful 12Sep2014 transformations
- 20:35, 27 September 2014 diff hist +6 Cloning 7 casein gBlocks, 12Sep2014 and on →Plasmid midipreps of successful 12Sep2014 transformations
- 20:32, 27 September 2014 diff hist +166 CLONE 082514 humKappaCasein(Kex+) →Experiment 082514_humKappaCasein(Kex+) Cloning
- 20:28, 27 September 2014 diff hist +149 Cloning strategy →Progress summary, milestones 1 & 2
- 20:21, 27 September 2014 diff hist +1 CLONE 082514 humKappaCasein(Kex+) →Experiment 082514_humKappaCasein(Kex+) Cloning
- 20:20, 27 September 2014 diff hist +1,335 CLONE 082514 humKappaCasein(Kex+) →Experiment 082514_humKappaCasein(Kex+) Cloning
- 20:03, 27 September 2014 diff hist +8 m Cloning 7 casein gBlocks, 12Sep2014 and on →Cloning casein gBlocks into pD1214 & transforming plasmids into E. coli
- 19:59, 27 September 2014 diff hist +52 Cloning 7 casein gBlocks, 12Sep2014 and on →Repeating Electra cloning and transformation for P.FAKS.humBeta.S
- 19:52, 27 September 2014 diff hist +67 CLONE 082514 humKappaCasein(Kex+) →Experiment 082514_humKappaCasein(Kex+) Cloning
- 17:43, 18 September 2014 diff hist +18 Cloning 7 casein gBlocks, 12Sep2014 and on →Plasmid midipreps of successful 12Sep2014 transformations
- 17:42, 18 September 2014 diff hist +354 Cloning 7 casein gBlocks, 12Sep2014 and on →Repeating Electra cloning and transformation for P.FAKS.humBeta.S
- 17:30, 18 September 2014 diff hist −21 Cloning 7 casein gBlocks, 12Sep2014 and on →Plasmid midipreps & DNA quantification of successful 12Sep2014 transformations
- 17:30, 18 September 2014 diff hist +79 Cloning 7 casein gBlocks, 12Sep2014 and on →Repeating Electra cloning and transformation for P.FAKS.humBeta.S
- 11:34, 17 September 2014 diff hist −5 Cloning strategy →Progress summary, milestones 1 & 2
- 11:32, 17 September 2014 diff hist +89 Cloning strategy →Progress summary, milestones 1 & 2
- 10:51, 17 September 2014 diff hist +63 m Cloning 7 casein gBlocks, 12Sep2014 and on →Plasmid midipreps & DNA quantification of successful 12Sep2014 transformations
- 10:37, 17 September 2014 diff hist +986 Cloning 7 casein gBlocks, 12Sep2014 and on
- 10:07, 17 September 2014 diff hist +1,762 Cloning 7 casein gBlocks, 12Sep2014 and on
- 23:07, 15 September 2014 diff hist −8 Cloning 7 casein gBlocks, 12Sep2014 and on
- 18:49, 15 September 2014 diff hist 0 m Cloning 7 casein gBlocks, 12Sep2014 and on
- 18:48, 15 September 2014 diff hist +96 Cloning 7 casein gBlocks, 12Sep2014 and on
- 18:46, 15 September 2014 diff hist +219 Cloning 7 casein gBlocks, 12Sep2014 and on
- 18:07, 15 September 2014 diff hist +48 Cloning strategy
- 17:59, 15 September 2014 diff hist +270 Cloning strategy
- 08:17, 13 September 2014 diff hist 0 Shared Laboratory Notebook
- 08:16, 13 September 2014 diff hist 0 Cloning 7 casein gBlocks, 12Sep2014 and on
- 08:16, 13 September 2014 diff hist 0 Shared Laboratory Notebook
- 08:15, 13 September 2014 diff hist +3,540 N Cloning 7 casein gBlocks, 12Sep2014 and on Created page with "== Experiment: Cloning 7 casein gBlocks, above, into pD1214, transforming into ''E. coli'', extracting and sequencing plasmid DNA== '''Overview:''' * During this series of e..."
- 07:48, 13 September 2014 diff hist +85 N File:Zymo E. coli competent cells transformation protocol.pdf This is the protocol accompanying the Zymo Research Mix & Go Competent E. coli cells. current
- 06:51, 13 September 2014 diff hist +48 Shared Laboratory Notebook
- 18:20, 8 September 2014 diff hist +12 m Cloning strategy
- 16:54, 6 September 2014 diff hist +23 CLONE 082514 humKappaCasein(Kex+)
- 16:48, 6 September 2014 diff hist +121 CLONE 082514 humKappaCasein(Kex+)
- 21:26, 5 September 2014 diff hist +62 Cloning strategy →Progress summary, milestones 1 & 2
- 10:13, 5 September 2014 diff hist +6 m Cloning strategy →Progress summary, milestones 1 & 2
- 09:58, 5 September 2014 diff hist −3 m Cloning strategy →Summary of progress, milestones 1 & 2
- 09:56, 5 September 2014 diff hist +1,101 Cloning strategy
- 08:32, 5 September 2014 diff hist +120 m CLONE 082514 humKappaCasein(Kex+) →Experiment 082514_humKappaCasein(Kex+) Cloning
- 08:17, 5 September 2014 diff hist +31 m CLONE 082514 humKappaCasein(Kex+) →Experiment 082514_humKappaCasein(Kex+) Cloning
- 07:40, 5 September 2014 diff hist +14 m CLONE 082514 humKappaCasein(Kex+) →Experiment 082514_humKappaCasein(Kex+) Cloning
- 07:36, 5 September 2014 diff hist −61 m CLONE 082514 humKappaCasein(Kex+) →Experiment 082514_humKappaCasein(Kex+) Cloning
- 07:31, 5 September 2014 diff hist +43 N File:D4025i.pdf Zymo Research's Zyppy Midiprep Kit protocol current
- 07:13, 5 September 2014 diff hist −9 m CLONE 082514 humKappaCasein(Kex+) →Experiment 082514_humKappaCasein(Kex+) Cloning
- 07:10, 5 September 2014 diff hist +108 m CLONE 082514 humKappaCasein(Kex+) →Experiment 082514_humKappaCasein(Kex+) Cloning
- 07:04, 5 September 2014 diff hist +93 N File:PD1214 Map.png Map of the pD1214 vector showing restriction sites, relevant elements and sequencing primers. current
- 07:00, 5 September 2014 diff hist +7 CLONE 082514 humKappaCasein(Kex+)
- 06:58, 5 September 2014 diff hist +1,267 CLONE 082514 humKappaCasein(Kex+) →Experiment 082514_humKappaCasein(Kex+) Cloning
- 05:47, 5 September 2014 diff hist +402 CLONE 082514 humKappaCasein(Kex+) →Experiment 082514_humKappaCasein(Kex+) Cloning
- 08:11, 2 September 2014 diff hist +2 CLONE 082514 humKappaCasein(Kex+) →Experiment 082514_humKappaCasein(Kex+) Cloning
- 04:53, 2 September 2014 diff hist +1,226 CLONE 082514 humKappaCasein(Kex+)
- 04:15, 2 September 2014 diff hist −91 Shared Laboratory Notebook
- 04:09, 2 September 2014 diff hist +91 Shared Laboratory Notebook
- 00:22, 2 September 2014 diff hist +61 CLONE 082514 humKappaCasein(Kex+) →Experiment 082514_humKappaCasein(Kex+) Cloning
- 01:25, 30 August 2014 diff hist +66 External communications
- 02:52, 26 August 2014 diff hist −1 Bureaucracy →ToDo
- 03:09, 6 August 2014 diff hist +93 External communications
- 03:06, 6 August 2014 diff hist +21 Working Groups
- 18:49, 4 August 2014 diff hist +104 m Working Groups