Difference between revisions of "Shared Laboratory Notebook"
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'''General Protocols''' | '''General Protocols''' | ||
*[[DNA Handling]] | *[[DNA Handling]] | ||
*[https://www.dna20.com/products/expression-vectors/electra-system#2 Electra cloning protocol] | |||
*[[Media:Zymo E. coli competent cells transformation protocol.pdf]] | |||
*[[Media:D4025i.pdf]] (Zyppy plasmid midiprep protocol) | |||
*[[Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf]] | |||
'''June 2014''' | '''June 2014''' | ||
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'''October 2014''' | '''October 2014''' | ||
*[[Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2]] | *[[Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2]] | ||
*[[Cloning human alpha casein S1, 04Oct2014 and on]] | |||
Revision as of 06:54, 6 October 2014
See also See http://2014.igem.org/Team:SF_Bay_Area_DIYbio/Notebook
General Protocols
- DNA Handling
- Electra cloning protocol
- Media:Zymo E. coli competent cells transformation protocol.pdf
- Media:D4025i.pdf (Zyppy plasmid midiprep protocol)
- Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf
June 2014
August 2014
September 2014
- Cloning 7 casein gBlocks, 12Sep2014 and on
- Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S
- Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014
- Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014
October 2014