Shared Laboratory Notebook

See also See http://2014.igem.org/Team:SF_Bay_Area_DIYbio/Notebook

General Protocols

• DNA Handling

June 2014

• CR_062214_humanKappaCasein_001

August 2014

• CLONE_082514_humKappaCasein(Kex+)
• Growth Curve Experiment

September 2014

• Cloning 7 casein gBlocks, 12Sep2014 and on
• Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S
• Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014
• Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014

October 2014

• Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2