User contributions for Rachel Linzer
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- 18:27, 6 October 2014 diff hist +25 m Real Vegan Cheese Notebook Documentation Guidelines
- 08:20, 6 October 2014 diff hist +295 N Real Vegan Cheese Notebook Training Sign-off Created page with "When you've read our Real Vegan Cheese Notebook Documentation Guidelines guidelines or attended a notebook training, please record your name and date training was complete..."
- 08:15, 6 October 2014 diff hist +3,133 N Real Vegan Cheese Notebook Documentation Guidelines Created page with "As important as it is to do experiments, it's just as important to document them. Not documenting or incompletely documenting laboratory work is like not having done it at all..."
- 07:13, 6 October 2014 diff hist +148 Shared Laboratory Notebook
- 07:08, 6 October 2014 diff hist +20 m Cloning human alpha casein S1, 04Oct2014 and on →Overview
- 07:07, 6 October 2014 diff hist +818 N Cloning human alpha casein S1, 04Oct2014 and on Created page with "=Cloning human alpha casein S1, plasmid DNA midiprep and DNA conc./purity= ==Overview== During these experiments, we will insert our final IDT gBlock, human alpha casein, into..."
- 06:54, 6 October 2014 diff hist +372 Shared Laboratory Notebook
- 06:43, 6 October 2014 diff hist −3 m Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2
- 06:38, 6 October 2014 diff hist −1 m Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2
- 06:36, 6 October 2014 diff hist +3,015 Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2
- 20:26, 3 October 2014 diff hist +1 Cloning strategy →Progress summary, Milestones 1 & 2
- 20:26, 3 October 2014 diff hist +30 Cloning strategy →Progress summary, Milestones 1 & 2
- 18:36, 2 October 2014 diff hist +157 m IGEM Team
- 08:26, 2 October 2014 diff hist 0 Cloning strategy →Progress summary, milestones 1 & 2
- 08:25, 2 October 2014 diff hist +166 m Cloning strategy →Progress summary, milestones 1 & 2
- 08:18, 2 October 2014 diff hist +457 Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014
- 06:36, 2 October 2014 diff hist +224 Cloning 7 casein gBlocks, 12Sep2014 and on →Cloning casein gBlocks into pD1214 & transforming plasmids into E. coli current
- 06:33, 2 October 2014 diff hist +127 CLONE 082514 humKappaCasein(Kex+) current
- 06:25, 2 October 2014 diff hist +227 Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 →Cloning casein & FAM20C gBlocks into pD1214 & transforming plasmids into E. coli
- 06:20, 2 October 2014 diff hist +1,058 Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014
- 06:09, 2 October 2014 diff hist +118 N File:Spec results 29Sep2014 midipreps.png UV spectrophotometer results for DNA quantification and purity analysis performed on 29Sep2014 midiprepped plasmid DNA current
- 05:41, 2 October 2014 diff hist −12 Cloning 7 casein gBlocks, 12Sep2014 and on →Cloning 7 casein gBlocks into pD1214, transforming into E. coli, extracting and sequencing plasmid DNA
- 05:40, 2 October 2014 diff hist +58 Cloning 7 casein gBlocks, 12Sep2014 and on →Plasmid midipreps of successful 12Sep2014 transformations
- 05:37, 2 October 2014 diff hist −118 Cloning 7 casein gBlocks, 12Sep2014 and on →Plasmid DNA quantification & purity spec readings for 14Sep2014 midipreps
- 05:34, 2 October 2014 diff hist 0 Cloning strategy →Progress summary, milestones 1 & 2
- 05:30, 2 October 2014 diff hist +48 Cloning strategy →Progress summary, milestones 1 & 2
- 03:34, 1 October 2014 diff hist +12 m Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014
- 00:13, 1 October 2014 diff hist +92 Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014
- 00:05, 1 October 2014 diff hist +56 m Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014
- 00:01, 1 October 2014 diff hist +512 Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014
- 23:53, 30 September 2014 diff hist +2,029 N Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014 Created page with "'''September 30, 2014: DNA pick-up by [http://sequetech.com/ Sequetech] to sequence 16 samples, detailed below''' *'''Location:''' BioCurious *'''Participants:''' Rachel (set..."
- 23:28, 30 September 2014 diff hist +160 N File:Samples details from Sequetech sequence pick-up, 30Sep2014.png This table contains sample name, ID, size, primer, DNA concentration and purity details for samples submitted to Sequetech for sequencing on September 30, 2014. current
- 23:26, 30 September 2014 diff hist +81 Shared Laboratory Notebook
- 11:14, 30 September 2014 diff hist +2,375 Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 →Cloning bovine beta casein and Fam20C Kex +/- gBlocks into pD1214, transforming into E. coli, extracting and sequencing plasmid DNA
- 10:20, 30 September 2014 diff hist +2,038 Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014
- 07:19, 30 September 2014 diff hist +122 Existing vegan cheeses →Opinions
- 07:13, 30 September 2014 diff hist +378 Existing vegan cheeses →Daiya
- 06:57, 30 September 2014 diff hist +39 Cloning strategy →Progress summary, milestones 1 & 2
- 03:59, 30 September 2014 diff hist +117 Cloning 7 casein gBlocks, 12Sep2014 and on →Plasmid DNA quantification & purity spec readings for 14Sep2014 midipreps
- 03:56, 30 September 2014 diff hist +19 Real Vegan Cheese →Project pages
- 03:56, 30 September 2014 diff hist −54 Shared Laboratory Notebook
- 03:55, 30 September 2014 diff hist +7,261 N GBlock design Created page with "=Codon Optimization, Restriction Analysis and Flanking Sequences for Casein and FAM20C Kinase Genes= ==Overview== In this section, we use published amino acid sequences for ou..."
- 02:34, 30 September 2014 diff hist 0 N File:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf current
- 19:22, 29 September 2014 diff hist +618 GBlock Design for Casein and FAM20C Kinase Genes →Disruption of Kex protease amino acid recognition sites current
- 19:03, 29 September 2014 diff hist +5,488 GBlock Design for Casein and FAM20C Kinase Genes →Overview
- 15:48, 29 September 2014 diff hist +28 m Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014 →Cloning casein & FAM20C gBlocks into pD1214 & transforming plasmids into E. coli
- 08:57, 29 September 2014 diff hist −3 m GBlock Design for Casein and FAM20C Kinase Genes →Overview
- 08:52, 29 September 2014 diff hist +1,158 N GBlock Design for Casein and FAM20C Kinase Genes Created page with "=Codon Optimization, Restriction Analysis and Flanking Sequences for Casein and FAM20C Kinase Genes= ==Overview== In this section, we describe using published amino acid seque..."
- 08:10, 29 September 2014 diff hist 0 m Shared Laboratory Notebook
- 08:09, 29 September 2014 diff hist +54 Shared Laboratory Notebook
