Difference between revisions of "Shared Laboratory Notebook"
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*[[Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2]] | *[[Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2]] | ||
*[[Cloning human alpha casein S1, 04Oct2014 and on]] | *[[Cloning human alpha casein S1, 04Oct2014 and on]] | ||
*[[Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing 10Oct2014]] | |||
Revision as of 23:44, 10 October 2014
See also See http://2014.igem.org/Team:SF_Bay_Area_DIYbio/Notebook
General Protocols
- DNA Handling
- Electra cloning protocol
- Media:Zymo E. coli competent cells transformation protocol.pdf
- Media:D4025i.pdf (Zyppy plasmid midiprep protocol)
- Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf
General Documentation Information
June 2014
August 2014
September 2014
- Cloning 7 casein gBlocks, 12Sep2014 and on
- Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S
- Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014
- Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014
October 2014