Difference between revisions of "Real Vegan Cheese"
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= More links to incorporate = | = More links to incorporate = | ||
*[[Science]] | *[[Science]] | ||
**[[Microbiology]] | **[[Microbiology]] | ||
**[[Protein Expression in Yeast]] | **[[Protein Expression in Yeast]] | ||
*[[Budget, planning and orders]] | *[[Budget, planning and orders]] | ||
*[[Bureaucracy]] | *[[Bureaucracy]] | ||
*[[Cheese making]] | *[[Cheese making]] | ||
*[[Existing vegan cheeses]] | *[[Existing vegan cheeses]] | ||
*[[Experiments started January 2016]] | *[[Experiments started January 2016]] | ||
Revision as of 03:26, 20 June 2023
The goal of this project is to make real vegan cheese by engineering normal baker's yeast (S. cerevisiae) to express milk protein (casein), purifying the protein, creating a milk-substitute by blending in vegan replacements for lactose and milkfat, and finally turning the resulting milk-substitute into semi-hard cheese like gouda using the normal cheese-making process. Watch a brief video about the project.
The main challenges include:
- Biology
- Find all the nitty-gritty on how we engineer our host organism to produce cheese proteins in our pages on molecular biology, cloning strategy, gBlock design, plasmid design, Yeast Transformation.
- Getting the major casein proteins expressed, and ideally secreted.
- Achieving a high level of expression.
- Ensuring any necessary post-translational modifications take place.
- Cost-efficient protein purification.
- Stable micelle-formation.
- Finding a suitable milk-fat replacement.
- Finding a suitable lactose replacement
- Can we achieve the same flavor and aroma profile using the traditional cheese ripening bacterial cultures?
- Ethical, Legal, and Social Implications
- A comparison between yeast bioreactors and dairy cows will be essential
- Can we produce this cheese in a way that is (a) better for the environment, and (b) economically feasible? Check our page on Life Cycle Assessments and Techno-Economic Analyses
- Safety
- Ensuring a safe and responsible research environment is already a key goal of CCL and BioC, but we will have to field potential safety concerns specifically related to the Real Vegan Cheese project.
- Check out the Community Biology Safety Handbook, edited with help from some of our members.
- Funding and spreading the word
- We have raised about $37,000 on indiegogo and the funding we raised is being used on developing the feasibility of producing Real Vegan Cheese from baker's yeast.
- Patents
- We plan to patent and abandon to keep this technology free for everyone
- Education
- Providing hands-on lab training.
- Check out our Educational Videos about many parts of the process!
Getting involved
So, you want to join the Real Vegan Cheese project? - Orientation for new folks - September 2022 blog post.
Here are some different ways to get involved:
- Go to one of our Monday evening working meetings 7pm PST:
- Read our Introductory mini-textbook (work in progress)
- Get on one or more of our mailing lists
- Help fund the project
- Send us an email at info@counterculturelabs.org
- Add to this wiki.
More links to incorporate
- Science
- Budget, planning and orders
- Bureaucracy
- Cheese making
- Existing vegan cheeses
- Experiments started January 2016
- External communications
- Finances - Incoming monies, purchases, etc.
- Health effects: allergy and cancer link
- How to take notes and record data
- Media - Articles written about us
- Product viability
- Narwhal Cheese!
- Questions and answers
- Service and reagent providers - Sequencing, synthesis, enzymes, etc.
- Shared Laboratory Notebook
- Similar projects - Other people doing similar stuff
- Software
- Video - Video and audio documentation, teleconferencing and live-streaming
- Wetware inventory