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Date Name Thumbnail Size User Description Versions
07:47, 12 August 2014 1-s2.0-S0022030209708653-gr1.jpg (file) 44 KB Patrik   2
05:39, 21 April 2020 14-07-28-2010-IMG 1020.jpg (file) 467 KB Patrik Casein team - assemble! 1
08:42, 12 August 2014 20110829170206GF p.pdf (file) 1.24 MB Patrik   2
05:03, 21 April 2020 20140728 225951.jpg (file) 693 KB Patrik We tried to dissolve casein powder in water in a couple different ways. One half of the test tubes was sonicated in a jewelry cleaner for about half an hour. One set of tubes was treated with one or two scoops of baking soda solution, until a pH of aro... 1
05:12, 21 April 2020 20140729 092606.jpg (file) 343 KB Patrik 2 scoops of baking soda solution, with or without sonication. The unsonicated casein on the right clearly settled out of solution overnight; the sonicated on the left is still nicely in solution 1
05:19, 21 April 2020 20140729 092641.jpg (file) 894 KB Patrik Unsonicated, baking soda vs citric acid + baking soda. 1
05:22, 21 April 2020 20140729 092710.jpg (file) 328 KB Patrik Sonicated controls - some settling, but not too much, even without any additions 1
05:26, 21 April 2020 20140729 092728.jpg (file) 696 KB Patrik Sonicated; baking soda only vs citric acid + baking soda: baking soda only looked marginally better in person; hard to see on the pics: 1
05:27, 21 April 2020 20140729 092844.jpg (file) 747 KB Patrik Unsonicated controls; this is what happens when you just try to dissolve casein powder in water and let it sit for an hour or two 1
08:16, 26 October 2014 5-FOA Protocol, Zymo Research.pdf (file) 276 KB Rachel Linzer Protocol for use of 5-FOA to select against URA3+ yeast strains. 1
08:00, 12 August 2014 Alpha-s1-casein.jpg (file) 20 KB Patrik   2
08:01, 12 August 2014 Alpha-s2-casein.jpg (file) 18 KB Patrik   2
01:40, 16 December 2014 Bannerv6.jpg (file) 0 bytes Patrik Banner for iGEM 2014 1
08:02, 12 August 2014 Beta-casein.jpg (file) 20 KB Patrik   2
07:01, 30 October 2014 CAM01681.jpg (file) 1.53 MB Patrik Lab benches 1
07:43, 30 October 2014 CAM01690.jpg (file) 1.67 MB Patrik ? 1
07:47, 30 October 2014 CAM01691.jpg (file) 1.55 MB Patrik Open on bench 1hr 1
07:52, 30 October 2014 CAM01692.jpg (file) 1.52 MB Patrik Yeast strains on URA- plates 1
07:54, 30 October 2014 CAM01693.jpg (file) 1.46 MB Patrik Various equipment 1
07:55, 30 October 2014 CAM01694.jpg (file) 1.62 MB Patrik Surfaces inside BioSafety Cabinet. 1
07:57, 30 October 2014 CAM01695.jpg (file) 1.49 MB Patrik Water sources - all clear! 1
07:59, 30 October 2014 CAM01696.jpg (file) 1.53 MB Patrik More lab benches - yuck! 1
02:34, 30 September 2014 Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf (file) 220 KB Rachel Linzer   1
07:31, 5 September 2014 D4025i.pdf (file) 170 KB Rachel Linzer Zymo Research's Zyppy Midiprep Kit protocol 1
20:19, 11 March 2015 DurringSonication.jpg (file) 1.92 MB Sam re-milk during sonication 1
09:07, 12 August 2014 ElectrapDAUGHTERinsertion.png (file) 136 KB Patrik Electra system pDAUGHTER insertion 1
19:10, 14 March 2015 FullCurdinReMilk.jpg (file) 135 KB Sam Nice curd formation in re-milk with CaCl added and pH reduced. 1
21:21, 16 October 2014 Function Align two seqs.png (file) 265 KB Craig   1
00:57, 20 January 2015 Gel2 17Jan2015 labeled.jpg (file) 47 KB Rachel Linzer   1
00:49, 20 January 2015 Gel2 17Jan2015 labeled.png (file) 2.51 MB Rachel Linzer Labeled Gel#2 from 17Jan2015 1
11:46, 8 September 2014 HumanKap(Kex+) Rev.png (file) 118 KB Craig Reverse direction sequencing. 3
11:49, 8 September 2014 HumanKappa(Kex+).png (file) 125 KB Craig   2
17:54, 30 August 2014 Human Kappa Casein Map.png (file) 71 KB Craig   1
08:03, 12 August 2014 Kappa-casein.jpg (file) 31 KB Patrik   2
02:14, 20 January 2015 LifeTech NuPAGE TrisGlycine Mini Gels Cat-EC6025BOX.pdf (file) 4.28 MB Rachel Linzer   1
09:23, 8 November 2022 Masked Narwhal2.png (file) 597 KB Patrik   1
21:14, 12 March 2015 MesophillicCulture.jpg (file) 195 KB Sam   1
02:18, 13 March 2015 MilkCurds1.jpg (file) 148 KB Sam Consistency of milk curds. 1
02:23, 13 March 2015 MilkCurds2.jpg (file) 154 KB Sam another shot of real milk curd. 1
02:16, 13 March 2015 MilkpHtest.jpg (file) 194 KB Sam Vial # 2 contains the milk before the addition of culture, the unlabeled vial contains the milk 1/2hr after addition of the culture. The vial on the far left is re-milk with acid added so that it would intentionally overshoot the range of the pH scale.... 1
00:51, 11 October 2014 Minutes of Initial Meeting of the Board of Directors Real Vegan Cheese 9.29.14.pdf (file) 169 KB Maria Chavez   1
06:18, 8 November 2022 NarwhalCheese.png (file) 204 KB Patrik   1
19:14, 14 March 2015 NoCurdpHChangeOnly.jpg (file) 130 KB Sam No evidence of curd formation was seen in the re-milk that only had its pH reduced. 1
21:19, 16 October 2014 Open forward.png (file) 369 KB Craig   1
05:54, 12 August 2014 PD1214-FAKS.png (file) 225 KB Patrik pD1214-FAKS Electra vector 1
07:04, 5 September 2014 PD1214 Map.png (file) 134 KB Rachel Linzer Map of the pD1214 vector showing restriction sites, relevant elements and sequencing primers. 1
21:16, 12 March 2015 PHmeasurement.jpg (file) 158 KB Sam Our pH 'meter' 1
03:56, 27 September 2022 PPIC35K-Ost1-Alpha-AS1-sequence.pdf (file) 34 KB Cyanobacterium File uploaded with MsUpload 1
03:58, 27 September 2022 PPIC35K-Ost1-Alpha-AS2-sequence.pdf (file) 33 KB Cyanobacterium File uploaded with MsUpload 1
04:02, 27 September 2022 PPIC35K-Ost1-Beta-sequence.pdf (file) 33 KB Cyanobacterium File uploaded with MsUpload 1
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