Difference between revisions of "SDS Page-Gel for all the bovine proteins"
Emi Nikolov (talk | contribs) |
Emi Nikolov (talk | contribs) |
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Participants: Wesley, Meenakshi, Nikola, Johan | Participants: Wesley, Meenakshi, Nikola, Johan | ||
Materials: | Materials: casein, 3B(100 fold diluted), 6B (100 fold diluted), 7 (100 fold diluted), 2B (100 fold diluted), control SFOA, transformed negative control, liquid culture, Bovine Beta B (8) - 20ml + 4ml loading dye, Bovine Alpha A (8) - 20ml + 4ml loading dye | ||
Method: | Method: | ||
Get 3 colonies from each plates in 1 centrifuge tube + resuspend into 20ml distilled water | |||
For casein touch 1 20-2w microliter pipette tip and rinse with 20 microliter dionised water in 1 casein control tube | |||
One sample take 1 colony from SFOA (as a negative control) | |||
One sample from control yeast transform plate | |||
To run the gel | |||
Take 20 microliter of sample and 4 microliter loading dye | |||
Boil all the samples for 10 minutes | |||
Put 400ml loading buffer in tank. | |||
(We have , so, 10ml in 90 ml distilled water.) | |||
Result: | Result: | ||
Revision as of 06:18, 25 November 2014
SDS Page-Gel for all of the bovine proteins
Monday Nov 24th
SDS Page-Gel for all of the bovine proteins
Participants: Wesley, Meenakshi, Nikola, Johan
Materials: casein, 3B(100 fold diluted), 6B (100 fold diluted), 7 (100 fold diluted), 2B (100 fold diluted), control SFOA, transformed negative control, liquid culture, Bovine Beta B (8) - 20ml + 4ml loading dye, Bovine Alpha A (8) - 20ml + 4ml loading dye
Method: Get 3 colonies from each plates in 1 centrifuge tube + resuspend into 20ml distilled water For casein touch 1 20-2w microliter pipette tip and rinse with 20 microliter dionised water in 1 casein control tube One sample take 1 colony from SFOA (as a negative control) One sample from control yeast transform plate
To run the gel Take 20 microliter of sample and 4 microliter loading dye Boil all the samples for 10 minutes Put 400ml loading buffer in tank. (We have , so, 10ml in 90 ml distilled water.)
Result: