IGEM team meeting notes 4/21/14

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iGEM planning 4/21/14 (copied from google docs https://docs.google.com/document/d/1wi2GV0_gS1eMfIRfp8WCTkkR5DFUve0BzrDy-dTALCE/edit?usp=sharing)

Constructs to attempt

DNA Source(s):

Bovine

Human

Narwahl - if available (UCSC?)

Yeasts:

https://wiki.counterculturelabs.org/index.php/Vegan_cheese/Molecular_biology#Host_organism

Pichia pastoris

Saccharomyces cerevesiae

Proteins:

kappa-casein: Craig, Patrik

https://www.warf.org/documents/technology-summary/P98198US.pdf

beta-casein: Ahnon, Fran

bovine seq: http://www.ncbi.nlm.nih.gov/nuccore/162804?report=genbank
human mRNA seq: http://www.ncbi.nlm.nih.gov/nuccore/288097?report=genbank

(human) Science Overview (include genetic variants & related health issues): http://www.betacasein.org/index.php?p=science-overview

alpha-s1: Ashley

(alpha-s2): Bryce

casein kinase protein? May need it for efficient phosphorylation - Marc

Craig will look into ordering from IDT -

Funding

CrowdTilt will end in 3 days - restart? Jing wants us to work more on the video:

“iGEM cheese project” shotbox file: http://beta.shotbox.se/stories/3082

Patrik: contact sponsors