SDS Page-Gel for all the bovine proteins

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SDS Page-Gel for all of the bovine proteins

Monday Nov 24th

SDS Page-Gel for all of the bovine proteins

Participants: Wesley, Meenakshi, Nikola, Johan

Materials:

Casein

3B(100 fold diluted)

6B (100 fold diluted)

7 (100 fold diluted)

2B (100 fold diluted)

control 5FOA

transformed negative control

liquid culture

Bovine Beta B (8) - 20ml + 4ml loading dye

Bovine Alpha A (8) - 20ml + 4ml loading dye

Method: Get 3 colonies from each plates in 1 centrifuge tube + resuspend into 20ml distilled water For casein touch 1 20-2w microliter pipette tip and rinse with 20 microliter dionised water in 1 casein control tube One sample take 1 colony from SFOA (as a negative control) One sample from control yeast transform plate

To run the gel Take 20 microliter of sample and 4 microliter loading dye Boil all the samples for 10 minutes Put 400ml loading buffer in tank. (We have , so, 10ml in 90 ml distilled water.)


Result:

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