Quantified IDT DNA (human kappa casein kex-) sample on nanodrop spectrophotometer to verify that the resuspension back in September worked.

November 06, 2014: Experiment to quantify the amount of DNA in the sample received from IDT

• Location: BioCurious

• Participants: Aaron Bryan, Johan

• Notes: Johan

• Aims: Before ordering the new electra kit, we wish to verify that we correctly suspending the IDT DNA.

• Materials:
  • Nanodrop Sequencer
  • 1 ul of human kappa casein kex- DNA received from IDT that was resuspended in 10ul of TE/H20 back in Sept. 2014
  • 1 ul of TE/H20 mixed in 1:1 ratio to duplicate resuspension buffer

• Methods:
  • 1 ul of 50% TE was used to blank the nanodrop. Following this 1ul of the human kappa casein kex- DNA was used to verify the amount of DNA present.

• Results:

The sample was found have a concentration of 17ng/ul of DNA.