IGEM team meeting notes 10/13/14

Meeting 10/13/2014 at CCL

Call-in info: https://zoom.us/j/820128495 Or, go to https://zoom.us/join and enter meeting ID: 820 128 495 hello is the zoom starting Attendees: Biocurious:

   Zoom: Advait, Maria, Johan
   CCL: Rebecca, Thorson, Jamie, Jim (retired plant molecular geneticist from UC Berkeley), Stan, Patrik, Rachel
   Home: Craig

Agenda

Remaining iGEM material due by midnight Friday Oct 17! Judging form due (Teams should highlight their best BioBrick Parts, and indicate which special prizes each team thinks they should be eligible for.) Giant Jamboree wiki FREEZE at 11:59PM EDT We need to port EVERYTHING to the iGEM wiki! Additional Part DNA due to the Registry (not required, however parts are not eligible for medals and awards) Do we want to use one of the iGEM templates? http://2014.igem.org/WikitemplateB_home or http://2014.igem.org/WikitemplateA_home Template B.by general consensus and for ease of use. Who will be working on which page PLEASE DOUBLE CHECK WITH THE PERSON WHO WILL BE EDITING THE PAGE WHEN UPDATING: Front page/ Official Team profile - Maria + Patrik Team - Maria (** STATUS DONE EXCEPT FOR SOME PEOPLE INFO MISSING **) Project - Rachel (technical parts) + Thorson + Maria (formatting etc) (include future plans, briefly) Parts - Patrik Include future plans section - Maria Modeling Notebook - Maria + Rachel + Johan Safety - laboratory biosafety and issues surrounding human consumption - Rebecca Ethical, legal, social Implications - Rebecca + Maria Attributions (individual people should start adding what they worked on themselves)

2. Work remaining on BioBricks parts. Patrik filled out database entry for bovine Kappa and Beta; could use more work (references, etc.): Beta: BBa_K1531004 http://parts.igem.org/Part:BBa_K1531004 Kappa: BBa_K1531005 http://parts.igem.org/Part:BBa_K1531005

3. Yeast expression What were the results of all our swabbing experiments last week? Did anyone actually record the outcome? 5-FOA - did anyone order it? Craig Ordered (and paid -$67.43- please reimburse from zymoresearch, 100x solution; 10/13/14- details added in the online ledger) How does it work? http://www.zymoresearch.com/buffers-solutions/chemicals/5-fluoroorotic-acid-5-foa in strains expressing the functional URA3 gene coding for orotine-5-monophosphate decarboxylase that is involved in the synthesis of uracil. Yeast strains that are phenotypically Ura+ become Ura- and 5-FOA(R) (resistant) after selection because 5FOA is toxic to cells - yeast with a functional Ura3 gene die in the presence of 5-FOA. Next steps for yeast work? Grow up yeast, use 5-FOA to kill Ura3+ cells.

4. Need RVC stickers and postcards for East Bay Mini Maker Faire next S. Anyone available to help staff the booth? http://ebmakerfaire.wordpress.com/ SIGN UP HERE: https://docs.google.com/document/d/1wPCypfr85mRLl9Lw-Hf2JoU6d3ZU2JL7J6v9VaZ05xs/edit What else should we display at the booth - bioreactor vessels? 3D printed proteins?

5. Question for Johan & any other BioCurians re: human kappa (kex -). I know that the transformation 0f the 9/12/2014 cloning reaction yielded only 2 colonies once and 0 colonies on a repeat, but have you repeated the Electra cloning for human kappa (kex -)? Might be an easy fix. They have done a new Electra reaction. Grew at room temperature. Few small colonies that didn't grow in LB carb. Bad Electra enzyme after multiple freeze thaws??

10ul of FAM Kek+ 9A or 9B AND 10A or 10B (FAM Kex -): 2 samples total THANKS!!! Just let Emi know where they are or send a photo (https://wiki.realvegancheese.org/index.php/Submitting_10_casein_and_FAM20C_in_pD1214_plasmids_for_sequencing_30Sep2014)