Educational Videos

Team videos

• RVC Youtube Channel
  • Real Vegan Cheese - Launch video
  • Ask a Biohacker: How can we stay up-to-date after you have completed the project successfully? What are your plans to promote the production?
  • Ask a Biohacker: Could specific chemicals be produced more cheaply using this technology?
  • Ask a Biohacker: Is it more ethical to like robots or dinosaurs?
  • Ask a Biohacker: Could a cow milk allergy be causing my migraines?
  • Ask a Biohacker: What sugars are you thinking of using to replace lactose?
  • Ask a Biohacker: Can this cheese be made economically viable?
  • Real Vegan Cheese iGEM 2014 presentation
  • Let's have a BLAST
• Other team videos:
  • Ben Rupert and The Real Vegan Cheese Project
  • Benjamin Rupert: Real Vegan Cheese
  • On Narwhal Cheese, the Evolution of Whales, and Imaginary Phylogeny of Unicorns

Genetic Engineering / Synthetic Biology

• Making Yeast produce Deer Milk and Egg Whites - Whose Gene is it Anyway
• Spider Beer - Making Yeast Produce Spider Silk - Project Overview 13:39
• Making Bacteria that are Part JELLYFISH! 29:10
• The Parts of a Plasmid 4:38

Molecular Biology techniques

• Cloning and plasmid assembly
  • A Brief Look at Cloning with Restriction Enzymes (SnapGene, 3:22; great background on restriction enzymes)
  • Introduction to Restriction Enzyme Cloning (Jake Wintermute, 7:11)
  • Traditional Cloning Workflow (NEB, 0:49)
  • Gene Cloning with the School of Molecular Bioscience Long! 22:22, lots of context for uses of gene cloning. Includes PCR, gel purification, transformation, plasmid prep, restriction digest
  • Introduction to Gibson Assembly (NEB, 2:06)
  • Gibson Assembly Workflow (NEB, 0:57)
  • A Brief Look at Golden Gate Cloning (SnapGene, 3:03)
    • Longer version by SnapGene: Introduction to Golden Gate Cloning (5:04) + A Detailed Look at Golden Gate Assembly (3:32)

• Bacterial transformations:
  • How to Transform E. coli by Heat Shock
  • Bacterial Transformations
• Plasmid miniprep:
  • How to Harvest Plasmid DNA with Minipreps
• Protein expression and purification
  • Protein Purification (13:43)
  • Prepping proteins for purification: a practical look at lysis, sonication, ultracentrifugation, etc. (22:11 - excellent, but long)
• His-tag purification
  • Protein expression and purification (10:46)
  • Protein purification (6:21)
• Ammonium sulfate precipitation:
  • Salting in and salting out | How does salting out happen? | what is salting in of proteins? (6:05)
  • Ammonium sulphate precipitation of Protein (3:08)
  • Protein Purification (2:16)

Sequencing technologies

• Sanger sequencing 6:29
• How does Sanger Sequencing Work? 3:27
• Next Generation Sequencing 1: Overview - Eric Chow 31:25
• Ion Torrent Next-generation Sequencing 3:23
• Introduction to nanopore sequencing 2:15

Bioinformatics

• Blast
  • How to Use BLAST for Finding and Aligning DNA or Protein Sequences
  • NCBI Blast Tutorial
  • Webinar: A Practical Guide to NCBI BLAST on the Web
• Synonymous vs nonsynonymous mutation
  • Searching for Natural Selection: dN/dS
  • Introduction to computer exercise: detection of selection
• Homologous genes
  • Evolution of Genes (Part 1) - Mutations, Duplications, & Transpositions
  • Homolog vs paralog vs ortholog vs analog in 4 minutes | Genetics for beginners
  • Orthology searches and OrthoMCL
  • Coronaviridae Webinar, Session VI, Ortholog Group Prediction

Related topics

• The Microbiology of Cheese
• Is this the END of MEAT? | Vegan Documentary 2018