Difference between revisions of "Shared Laboratory Notebook"

From Real Vegan Cheese
Jump to navigation Jump to search
 
(11 intermediate revisions by 4 users not shown)
Line 1: Line 1:
See also See http://2014.igem.org/Team:SF_Bay_Area_DIYbio/Notebook
See also See http://2014.igem.org/Team:SF_Bay_Area_DIYbio/Notebook
'''For experiments from January 2016 on, see [[Experiments started January 2016]]'''


'''General Protocols'''
'''General Protocols'''
*[[DNA Handling]]
*[[DNA Handling]]
*[https://www.dna20.com/products/expression-vectors/electra-system#2 Electra cloning protocol]
*[https://www.dna20.com/products/expression-vectors/electra-system#2 Electra cloning protocol] (Used for cloning IDT gBlocks into plasmid backbone)
*[[Media:Zymo E. coli competent cells transformation protocol.pdf]]
*[[Media:Zymo E. coli competent cells transformation protocol.pdf]] (Protocol to transform plasmid DNA into chemically competent ''E. coli'')
*[[Media:D4025i.pdf]] (Zyppy plasmid midiprep protocol)
*[[Media:D4025i.pdf]] (Zyppy plasmid midiprep protocol; used to purify plasmid DNA from ''E.  coli'')
*[[Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf]]
*[[Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf]] (Protocol to transform plasmid DNA into yeast cells)
*[[Sequencing Analysis using Serial Cloner 2-6-1]]
*[[Sequencing Analysis using Serial Cloner 2-6-1]]
*[[Media:5-FOA Protocol, Zymo Research.pdf]] (Using 5-FOA to select against URA3+ yeast strains)
*[[Media:5-FOA Protocol, Zymo Research.pdf]] (Using 5-FOA to select against URA3+ yeast strains)
*[[Media:LifeTech_NuPAGE_TrisGlycine_Mini_Gels_Cat-EC6025BOX.pdf‎]] (General protocol for precast Tris-Glycine 4%-20% acrylamide protein gels)


'''General Documentation Information'''
'''General Documentation Information'''
Line 38: Line 41:
*[[Submitting FAM20C (Kex + & Kex -) for sequencing with new internal primers 14Oct2014]]
*[[Submitting FAM20C (Kex + & Kex -) for sequencing with new internal primers 14Oct2014]]
*[[Submitting FAM20C Kex + clone 9A and bovineKappa and bovineBeta: pSB1C3 Biobrick for sequencing 15Oct2014]]
*[[Submitting FAM20C Kex + clone 9A and bovineKappa and bovineBeta: pSB1C3 Biobrick for sequencing 15Oct2014]]
*[[Electra cloning of #5 Casein insert 22Oct2014]]
*[[Experiment to determine whether yeast is contaminated and to salvage strain using 5FOA, 23Oct2014]]
*[[Experiment to determine whether yeast is contaminated and to salvage strain using 5FOA, 23Oct2014]]
*[[Test yeast transformation Bovine alpha S1 and Bovine beta B caseins 26Oct2014]]
*[[Test yeast transformation Bovine alpha S1 and Bovine beta B caseins 26Oct2014]]
Line 54: Line 58:
'''December 2014'''
'''December 2014'''
*[[Repeat SDS Page-Gel for all the bovine proteins]] 07Dec2014
*[[Repeat SDS Page-Gel for all the bovine proteins]] 07Dec2014
'''January 2015'''
*[[Yeast Transformation of All Bovine Casein Inserts]] 10Jan2015
*[[SDS Page-Gel for all the bovine casein inserts]] 17Jan2015
'''May 2015'''
*[[Western Blot of bovine casein]] 2May2015
'''May 2016'''
*[[Plating URA- and Turbo Yeast Control]] 9-10 May 2016
'''June 2016'''

Latest revision as of 02:49, 15 June 2016

See also See http://2014.igem.org/Team:SF_Bay_Area_DIYbio/Notebook

For experiments from January 2016 on, see Experiments started January 2016

General Protocols

General Documentation Information

June 2014

August 2014

September 2014

October 2014

November 2014

December 2014

January 2015

May 2015

May 2016

June 2016