Difference between revisions of "Shared Laboratory Notebook"

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See also See http://2014.igem.org/Team:SF_Bay_Area_DIYbio/Notebook
See also See http://2014.igem.org/Team:SF_Bay_Area_DIYbio/Notebook


'''For experiments from January 2016 on, see [[Experiments started January 2016]]'''
'''General Protocols'''
*[[DNA Handling]]
*[[DNA Handling]]
*[https://www.dna20.com/products/expression-vectors/electra-system#2 Electra cloning protocol] (Used for cloning IDT gBlocks into plasmid backbone)
*[[Media:Zymo E. coli competent cells transformation protocol.pdf]] (Protocol to transform plasmid DNA into chemically competent ''E. coli'')
*[[Media:D4025i.pdf]] (Zyppy plasmid midiprep protocol; used to purify plasmid DNA from ''E.  coli'')
*[[Media:Clontech UTF-8'en-us'Quick Easy Yeast Transformation Mix Protocol-At-A-Glance 040214.pdf]] (Protocol to transform plasmid DNA into yeast cells)
*[[Sequencing Analysis using Serial Cloner 2-6-1]]
*[[Media:5-FOA Protocol, Zymo Research.pdf]] (Using 5-FOA to select against URA3+ yeast strains)
*[[Media:LifeTech_NuPAGE_TrisGlycine_Mini_Gels_Cat-EC6025BOX.pdf‎]] (General protocol for precast Tris-Glycine 4%-20% acrylamide protein gels)
'''General Documentation Information'''
*[[Real Vegan Cheese Notebook Documentation Guidelines]]
*[[Real Vegan Cheese Notebook Training Sign-off]]
'''June 2014'''
*[[CR_062214_humanKappaCasein_001]]
*[[CR_062214_humanKappaCasein_001]]
'''August 2014'''
*[[CLONE_082514_humKappaCasein(Kex+)]]
*[[CLONE_082514_humKappaCasein(Kex+)]]
*[[Growth Curve Experiment]]
'''September 2014'''
*[[Cloning 7 casein gBlocks, 12Sep2014 and on]]
*[[Cloning 7 casein gBlocks, 12Sep2014 and on]]
*[[Growth Curve Experiment]]
*[[Troubleshooting failed 12Sep2014 transformation of P.FAKS.humBeta.S]]
*[[Cloning bovine beta casein, Fam20C (Kex + & -), from 27Sep2014]]
*[[Submitting 10 casein and FAM20C in pD1214 plasmids for sequencing 30Sep2014]]
 
'''October 2014'''
*[[Initial studies of yeast strains' growth conditions and troubleshooting]]
*[[Repeat Midipreps to select new clones for sequencing for failed sequences from Oct. 2]]
*[[Cloning human alpha casein S1, 04Oct2014 and on]]
*[[Checking for contamination in yeast strains, media, water, and lab surfaces 06Oct2014]]
*[[Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit 08Oct2014]]
*[[Submitting 4 repeat and 1 new casein in pD1214 plasmid for sequencing 10Oct2014]]
*[[Cloning bovineKappa and bovineBeta into pSB1C3 Biobrick using NEB biobrick kit 10Oct2014]]
*[[Cloning bovineBeta into pSB1C3 Biobrick using NEB biobrick kit 13Oct2014]]
*[[Submitting FAM20C (Kex + & Kex -) for sequencing with new internal primers 14Oct2014]]
*[[Submitting FAM20C Kex + clone 9A and bovineKappa and bovineBeta: pSB1C3 Biobrick for sequencing 15Oct2014]]
*[[Electra cloning of #5 Casein insert 22Oct2014]]
*[[Experiment to determine whether yeast is contaminated and to salvage strain using 5FOA, 23Oct2014]]
*[[Test yeast transformation Bovine alpha S1 and Bovine beta B caseins 26Oct2014]]
 
'''November 2014'''
*[[Streaked new colonies of Bovine alpha S1 and Bovine beta B casein yeast transformations 03Nov2014]]
*[[Quantified IDT DNA (human kappa casein kex-) sample on nanodrop spectrophotometer to verify that the resuspension back in September worked.]] 06Nov2014
*[[Checked for colony growth in 03Nov2014 streak plates. Result: Yes]] 06Nov2014
*[[Making liquid cultures, in duplicate, of bovine Alpha S1 and bovine Beta B transformed yeast colonies.]] 12Nov2014
*[[Attempt SDS Page and also mini prep to confirm that yeast transformations worked]] 14Nov2014
*[[Repeat Mini Prep of bovine alpha S1 and bone beta B yeast]] 17Nov2014
*[[Yeast Transformation (Clontech kit)]] 18Nov2014
*[[SDS Page-Gel for all the bovine proteins]] 24Nov2014
*[[New 5 FOA Plates and prepare media]] 25Nov2014
 
'''December 2014'''
*[[Repeat SDS Page-Gel for all the bovine proteins]] 07Dec2014
 
'''January 2015'''
*[[Yeast Transformation of All Bovine Casein Inserts]] 10Jan2015
*[[SDS Page-Gel for all the bovine casein inserts]] 17Jan2015
 
'''May 2015'''
*[[Western Blot of bovine casein]] 2May2015
 
'''May 2016'''
 
*[[Plating URA- and Turbo Yeast Control]] 9-10 May 2016
 
'''June 2016'''

Latest revision as of 02:49, 15 June 2016

See also See http://2014.igem.org/Team:SF_Bay_Area_DIYbio/Notebook

For experiments from January 2016 on, see Experiments started January 2016

General Protocols

General Documentation Information

June 2014

August 2014

September 2014

October 2014

November 2014

December 2014

January 2015

May 2015

May 2016

June 2016