Difference between revisions of "Real Vegan Cheese"
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The goal of this project is to make real vegan cheese by engineering normal baker's yeast (S. cerevisiae) to express milk protein (casein), purifying the protein, creating a milk-substitute by blending in vegan replacements for lactose and milkfat, and finally turning the resulting milk-substitute into semi-hard cheese like gouda using the normal cheese-making process. Watch a [https://www.indiegogo.com/projects/real-vegan-cheese/ brief video about the project]. | |||
The main challenges include: | The main challenges include: | ||
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* Funding and spreading the word | * Funding and spreading the word | ||
** We | ** We have raised about $37,000 on indiegogo and the funding we raised is being used on developing the feasibility of producing Real Vegan Cheese from baker's yeast. | ||
* Patents | * Patents | ||
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Here are some different ways to get involved: | Here are some different ways to get involved: | ||
*Read our [[Introductory textbook|Introductory mini-textbook]] | *Go to one of our Monday evening working meetings 7pm PST: | ||
**[https://www.meetup.com/counter-culture-labs/events/calendar/ Counter Culture Labs Meetup calendar] | |||
**[https://www.meetup.com/biocurious/events/calendar/ BioCurious Meetup calendar] | |||
*Read our [[Introductory textbook|Introductory mini-textbook]] (work in progress) | |||
*Get on one or more of our [[mailing lists]] | *Get on one or more of our [[mailing lists]] | ||
*[https://realvegancheese.org/contribute/ Help fund the project] | *[https://realvegancheese.org/contribute/ Help fund the project] | ||
*Send us an email at info@counterculturelabs.org | *Send us an email at info@counterculturelabs.org | ||
* | *[https://www.realvegancheese.org/news/so-you-want-to-join-the-real-vegan-cheese-project-orientation-for-new-folks So, you want to join the Real Vegan Cheese project Blog? - 2022 Orientation for new folks] | ||
*Add to this wiki. | *Add to this wiki. | ||
= Project | = Project topics = | ||
*[[Science]] | |||
**[[Molecular biology]] | |||
**[[Microbiology]] | |||
**[[Cloning strategy]] | |||
**[[gBlock design]] | |||
**[[Yeast Transformation]] | |||
**[[Protein Expression in Yeast]] | |||
**[[Plasmid design]] | |||
**[[Bacterial cultures]] | |||
**[[Educational Videos]] | |||
*[[Narwhal Cheese]]! | |||
*[[Experiments started January 2016]] | |||
*[[Shared Laboratory Notebook]] | *[[Shared Laboratory Notebook]] | ||
*[[How to take notes and record data]] | |||
*[[Questions and answers]] | *[[Questions and answers]] | ||
*[[Cheese making]] | *[[Cheese making]] | ||
*[[Existing vegan cheeses]] | *[[Existing vegan cheeses]] | ||
*[[Ethical, Legal, and Social Implications]] | *[[Ethical, Legal, and Social Implications]] | ||
*[[Health effects and cancer link]] | *[[Health effects: allergy and cancer link]] | ||
*[[Product viability]] | *[[Product viability]] | ||
*[[Budget, planning and orders]] | *[[Budget, planning and orders]] | ||
Line 60: | Line 70: | ||
*[[Video]] - Video and audio documentation, teleconferencing and live-streaming | *[[Video]] - Video and audio documentation, teleconferencing and live-streaming | ||
*[[Finances]] - Incoming monies, purchases, etc. | *[[Finances]] - Incoming monies, purchases, etc. | ||
*[[Media]] - Articles written about us | |||
*[[Similar projects]] - Other people doing similar stuff | |||
*[[ |
Revision as of 04:43, 8 November 2022
The goal of this project is to make real vegan cheese by engineering normal baker's yeast (S. cerevisiae) to express milk protein (casein), purifying the protein, creating a milk-substitute by blending in vegan replacements for lactose and milkfat, and finally turning the resulting milk-substitute into semi-hard cheese like gouda using the normal cheese-making process. Watch a brief video about the project.
The main challenges include:
- Biology
- Getting the major casein-proteins secreted.
- Achieving a high level of expression.
- Ensuring the required post-translational modifications take place.
- Cost-efficient protein purification.
- Stable micelle-formation.
- Finding a suitable milk-fat replacement.
- Finding a suitable lactose replacement
- Ethical and environmental impact studies
- A comparison between yeast bioreactors and dairy cows will be essential
- Safety
- Ensuring a safe and responsible research environment is already a key goal of CCL and BioC, but we will have to field potential safety concerns specifically related to the Real Vegan Cheese project.
- Funding and spreading the word
- We have raised about $37,000 on indiegogo and the funding we raised is being used on developing the feasibility of producing Real Vegan Cheese from baker's yeast.
- Patents
- We plan to patent and abandon to keep this technology free for everyone
- Education
- Providing hands-on lab training.
Getting involved
Here are some different ways to get involved:
- Go to one of our Monday evening working meetings 7pm PST:
- Read our Introductory mini-textbook (work in progress)
- Get on one or more of our mailing lists
- Help fund the project
- Send us an email at info@counterculturelabs.org
- So, you want to join the Real Vegan Cheese project Blog? - 2022 Orientation for new folks
- Add to this wiki.
Project topics
- Science
- Narwhal Cheese!
- Experiments started January 2016
- Shared Laboratory Notebook
- How to take notes and record data
- Questions and answers
- Cheese making
- Existing vegan cheeses
- Ethical, Legal, and Social Implications
- Health effects: allergy and cancer link
- Product viability
- Budget, planning and orders
- Wetware inventory
- Software
- External communications
- Service and reagent providers - Sequencing, synthesis, enzymes, etc.
- Bureaucracy
- Video - Video and audio documentation, teleconferencing and live-streaming
- Finances - Incoming monies, purchases, etc.
- Media - Articles written about us
- Similar projects - Other people doing similar stuff