Difference between revisions of "Plasmid design"

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The following PDFs map the pPIC3.5K plasmid with each of the bovine casein genes inserted.
The following PDFs map the pPIC3.5K plasmid with each of the bovine casein genes inserted.
[[:File:pPIC35K-Ost1-Alpha-AS1-sequence.pdf]]


[[:File:pPIC35K-Ost1-Alpha-AS2-sequence.pdf]]
[[:File:pPIC35K-Ost1-Alpha-AS2-sequence.pdf]]
[[:File:pPIC35K-Ost1-Beta-sequence.pdf]]
[[:File:pPIC35K-Ost1-Beta-sequence.pdf]]
[[:File:pPIC35K-Ost1-Kappa-sequence.pdf]]
[[:File:pPIC35K-Ost1-Kappa-sequence.pdf]]
[[:File:pPIC35K-Ost1-Alpha-AS1-sequence.pdf]]


We then used Benchling to optimize the codons for expression in the yeast Pichia pastori.
We then used Benchling to optimize the codons for expression in the yeast Pichia pastori.

Latest revision as of 04:12, 27 September 2022

We’ve chosen EcoRI and NotI as our restriction enzymes, because they cut only in the right places on our plasmid.

The following PDFs map the pPIC3.5K plasmid with each of the bovine casein genes inserted.

File:pPIC35K-Ost1-Alpha-AS1-sequence.pdf

File:pPIC35K-Ost1-Alpha-AS2-sequence.pdf

File:pPIC35K-Ost1-Beta-sequence.pdf

File:pPIC35K-Ost1-Kappa-sequence.pdf

We then used Benchling to optimize the codons for expression in the yeast Pichia pastori.

Protease problems

See the protease problems page.