Difference between revisions of "Educational Videos"
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** [https://www.youtube.com/watch?v=tlVbf5fXhp4 Introduction to Gibson Assembly] (NEB, 2:06) | ** [https://www.youtube.com/watch?v=tlVbf5fXhp4 Introduction to Gibson Assembly] (NEB, 2:06) | ||
** [https://www.youtube.com/watch?v=2B_KaRv9NwU Gibson Assembly Workflow] (NEB, 0:57) | ** [https://www.youtube.com/watch?v=2B_KaRv9NwU Gibson Assembly Workflow] (NEB, 0:57) | ||
** [https://www.youtube.com/watch?v=GnI7uRn07RA A Brief Look at Golden Gate Cloning] (SnapGene, 3:03)* Bacterial transformations: | ** [https://www.youtube.com/watch?v=GnI7uRn07RA A Brief Look at Golden Gate Cloning] (SnapGene, 3:03) | ||
*** Longer version by SnapGene: [https://www.youtube.com/watch?v=aBcqev1NMMo Introduction to Golden Gate Cloning] (5:04) + [https://www.youtube.com/watch?v=NzQdLQ44I7w A Detailed Look at Golden Gate Assembly] (3:32) | |||
* Bacterial transformations: | |||
** [https://www.youtube.com/watch?v=gkjj9AypuFQ How to Transform E. coli by Heat Shock] | ** [https://www.youtube.com/watch?v=gkjj9AypuFQ How to Transform E. coli by Heat Shock] | ||
** [https://www.youtube.com/watch?v=1PqWnu2uBrY Bacterial Transformations] | ** [https://www.youtube.com/watch?v=1PqWnu2uBrY Bacterial Transformations] | ||
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** [https://www.youtube.com/watch?v=rom85WMAm08 Protein Purification] | ** [https://www.youtube.com/watch?v=rom85WMAm08 Protein Purification] | ||
** [https://www.youtube.com/watch?v=gnhUh6qVD5Y Salting in and salting out | How does salting out happen? | what is salting in of proteins?] | ** [https://www.youtube.com/watch?v=gnhUh6qVD5Y Salting in and salting out | How does salting out happen? | what is salting in of proteins?] | ||
= Sequencing teschnologies = | |||
* [https://www.youtube.com/watch?v=KTstRrDTmWI Sanger sequencing] 6:29 | |||
* [https://www.youtube.com/watch?v=e2G5zx-OJIw How does Sanger Sequencing Work?] 3:27 | |||
* [https://www.youtube.com/watch?v=mI0Fo9kaWqo Next Generation Sequencing 1: Overview - Eric Chow] 31:25 | |||
* [https://www.youtube.com/watch?v=zBPKj0mMcDg Ion Torrent Next-generation Sequencing] 3:23 | |||
* [https://www.youtube.com/watch?v=qzusVw4Dp8w Introduction to nanopore sequencing] 2:15 | |||
= Bioinformatics = | = Bioinformatics = | ||
Revision as of 22:20, 9 December 2022
Team videos
- RVC Youtube Channel
- Real Vegan Cheese - Launch video
- Ask a Biohacker: Question #1
- Ask a Biohacker: Question #2
- Ask a Biohacker: Question #3 Is it more ethical to like robots or dinosaurs?
- Ask a Biohacker: Question #4
- Ask a Biohacker: Question #5
- Ask a Biohacker: Question #6
- Real Vegan Cheese iGEM 2014 presentation
- Let's have a BLAST
- Other team videos:
Genetic Engineering / Synthetic Biology
- Making Yeast produce Deer Milk and Egg Whites - Whose Gene is it Anyway
- Spider Beer - Making Yeast Produce Spider Silk - Project Overview 13:39
- Making Bacteria that are Part JELLYFISH! 29:10
- The Parts of a Plasmid 4:38
Molecular Biology techniques
- Cloning and plasmid assembly
- A Brief Look at Cloning with Restriction Enzymes (SnapGene, 3:22; great background on restriction enzymes)
- Introduction to Restriction Enzyme Cloning (Jake Wintermute, 7:11)
- Traditional Cloning Workflow (NEB, 0:49)
- Gene Cloning with the School of Molecular Bioscience Long! 22:22, lots of context for uses of gene cloning. Includes PCR, gel purification, transformation, plasmid prep, restriction digest
- Introduction to Gibson Assembly (NEB, 2:06)
- Gibson Assembly Workflow (NEB, 0:57)
- A Brief Look at Golden Gate Cloning (SnapGene, 3:03)
- Longer version by SnapGene: Introduction to Golden Gate Cloning (5:04) + A Detailed Look at Golden Gate Assembly (3:32)
- Bacterial transformations:
- Plasmid miniprep:
- Protein expression and purification
- Ammonium sulfate precipitation:
Sequencing teschnologies
- Sanger sequencing 6:29
- How does Sanger Sequencing Work? 3:27
- Next Generation Sequencing 1: Overview - Eric Chow 31:25
- Ion Torrent Next-generation Sequencing 3:23
- Introduction to nanopore sequencing 2:15
Bioinformatics
- Blast
- Synonymous vs nonsynonymous mutation
- Homologous genes