Difference between revisions of "Cloning human alpha casein S1, 04Oct2014 and on"
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(Created page with "=Cloning human alpha casein S1, plasmid DNA midiprep and DNA conc./purity= ==Overview== During these experiments, we will insert our final IDT gBlock, human alpha casein, into...") |
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=Cloning human alpha casein S1, plasmid DNA midiprep and DNA conc./purity= | =Cloning human alpha casein S1, plasmid DNA midiprep and DNA conc./purity= | ||
==Overview== | ==Overview== | ||
During these experiments, we will insert our final IDT gBlock, human alpha casein, into the pD1214 vector and transform our constructs into ''E. coli.'' We will then perform midiprep plasmid DNA extractions, quantify our plasmid DNA and submit plasmid DNA from duplicate clones for sequencing. Sequence-confirmed DNA is then ready to be transformed into yeast. | During these experiments, we will insert our final IDT gBlock, human alpha casein (received 03Oct2014,) into the pD1214 vector and transform our constructs into ''E. coli.'' We will then perform midiprep plasmid DNA extractions, quantify our plasmid DNA and submit plasmid DNA from duplicate clones for sequencing. Sequence-confirmed DNA is then ready to be transformed into yeast. | ||
== Cloning casein & FAM20C gBlocks into pD1214 == | == Cloning casein & FAM20C gBlocks into pD1214 == | ||
Revision as of 07:08, 6 October 2014
Cloning human alpha casein S1, plasmid DNA midiprep and DNA conc./purity
Overview
During these experiments, we will insert our final IDT gBlock, human alpha casein (received 03Oct2014,) into the pD1214 vector and transform our constructs into E. coli. We will then perform midiprep plasmid DNA extractions, quantify our plasmid DNA and submit plasmid DNA from duplicate clones for sequencing. Sequence-confirmed DNA is then ready to be transformed into yeast.
Cloning casein & FAM20C gBlocks into pD1214
October 4, 2014
- Participants: Meenakshi, Johan, Lafia
- Notes:
- Location: BioCurious
Transforming human alpha casein S1:pD1214 cloning reaction product into E. coli
October 4, 2014
- Participants: Lafia, Meenakshi, Johan
- Notes:
- Location: BioCurious