2017 experiments

1/23/2017

We autoclaved three different agar media and poured plates:

Nutrient Agar:

• 2 g Carolina Nutrient Broth Mix
• 2.5 g Agar
• 250 ml distilled water

CCL1:

• 6.3 g soy powder
• 0.63 g K2 HPO4
• 1.56 g NaOAc
• 0.055 g magnesium chloride
• 0.016 manganese chloride
• 2.5 g agar
• 6.25 glucose
• 270 ml distilled water

MSY

• 2% soy powder
• 0.5% yeast extract
• 1.5% agar

CCL1 overflowed in the autoclave, so we were only able to pour the MSY and Nutrient Broth plates.

We also inoculated bacterial cultures on 2% skim milk (Berkeley Farms DairyPure), from freezedried commercial cultures:

1. Streptococcus thermophilus
2. Streptococcus salivarius ssp. thermophilus
3. Lactobacillus paracasei

1/30/2017

We checked on the three milk cultures from last week. All of them had solidified to yogurt. S. salivarius had grown mold in it, so we started a new culture. We streaked plates for isolates, but found the agar was too soft and it was difficult to avoid puncturing the surface with the inoculation loops while streaking. Also, we streaked them directly from the dairy cultures, as we did not have liquid media to wash the cells with.