2017 experiments

1/23/2017

We autoclaved three different agar media and poured plates:

Nutrient Agar:

• 2 g Carolina Nutrient Broth Mix
• 2.5 g Agar
• 250 ml distilled water

CCL1:

• 6.3 g soy powder
• 0.63 g K2 HPO4
• 1.56 g NaOAc
• 0.055 g magnesium chloride
• 0.016 manganese chloride
• 2.5 g agar
• 6.25 glucose
• 270 ml distilled water

MSY

• 2% soy powder
• 0.5% yeast extract
• 1.5% agar

We also inoculated bacterial cultures on 2% skim milk (Berkeley Farms DairyPure), from freezedried commercial cultures:

1. Streptococcus thermophilus
2. Streptococcus salivarius ssp. thermophilus
3. Lactobacillus paracasei