SDS Page-Gel for all the bovine casein inserts

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17 January 2015: SDS Page-Gel All 5 Bovine Casein Inserts

Location: BioCurious

Participants: Lafia, Johan, Meenakshi, Emi, Rachel

Notes: Emi

Aims: To see if we have casein expression bands of supernatant

Materials

Gel loading tips

Tris-Acelate SDS Running Buffer novex life tech

Bio-Rad pipette tips

Native gels... (need details)

ladder 20x

Casein

Cytosin


Protocol

Centrifuge the falcon tubes, 5 minutes 2800xg

Take 500ul of distilled water in eppendorf tube + 1 tiny drop of casein

Take 15ul of chymosin +1 tiny drop of casein in 250ul of water

Take 250ul of 7F (Bov Kappa) + 15ul of Chymosin

Take 40ul of 1, 8, 3B, 6B, +7F also the negative control to eppendorf tubes

Preparation fo 20x running buffer

Pour 25ul of 20x running buffer; make it to 500ul by adding the distilled water

5ul of loading dye to each tubes + 40ul of sample

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