Making liquid cultures, in duplicate, of bovine Alpha S1 and bovine Beta B transformed yeast colonies.

Wednesday Nov 12th

Participants: Meenakshi, Lafia, Nikola, Johan,

Objective: Make liquid cultures, in duplicate, of bovine Alpha S1 and bovine Beta B transformed yeast colonies.

Materials: 1 plate of transformed bovine Alpha S1 1 plate of transformed bovine Beta B 1 plate of untransformed 5FOA media colonies (control) 1 bag Powder CM URA- media. 6 50 ml tubes with 20 ml of URA- media

Method: Prepared 250 ml of URA- media by mixing 7g of URA- CM powder in millipore water. Mixture was autoclaved. Hood was prepped with sterile technique. UV for 15 minutes, followed by chlorine and alcohol wipe downs. A colony of bovine Alpha S1 was selected and inoculated into a 50 ml tube.A colony of bovine Beta B was selected and inoculated into a 50 ml tube. A colony from the untransformed 5FOA yeast plate was selected and inoculated into a 50 ml tube. These steps were repeated so that we would have duplicates (ie, two tubes made from each plate for a total of 6).

The tubes were placed in the 30 degree incubator.