IGEM team meeting notes 9/29/14

Meeting 9/29/2014

regular RVC meeting and first ever board meeting at CCL; experiments being finished up at BioC

Attendees: ? BioCurious: Maria, Lafia, Meenakshi, Nikola, Advait, Rachel, Emi, Johan, Aaron, Matt CCL: Patrik, Stan, Thorson, Craig, Marc, Carl Annoucenements:

Agenda

Initial board of directors meeting https://pad.riseup.net/p/vegancheeseboard Finances update https://wiki.realvegancheese.org/index.php/Finances

Perk fulfilment update

Planning next experiments/experiment update: https://wiki.realvegancheese.org/index.php/Cloning_strategy#Progress_summary.2C_milestones_1_.26_2 Troubleshooting Electra (-) control plate colonies from 27Sep2014 transformation Plate electra reagents alone (diluted in ~100uL water - plate whole reaction). post all upcoming protocols on wiki & step through them to see what we need do we have everything we need for yeast experiments? Strains yes Kit yes Media? No, on its way Plasmid DNA caseins & FAM20C:pD1214: midiprep stocks should be concentrated enough for several reactions. do we have everything we need to run SDS-PAGE gels? SDS Buffer? Unknown. BioC team will do inventory tonight Nupage Novex

do we have everything we need to clone into iGEM storage vector? Need to order restriction enzymes? Patrik will check BioBrick Part DNA due at the Registry on October 10 - only one weekend left! http://parts.igem.org/DNA_Submission

Board meeting pad: https://pad.riseup.net/p/vegancheeseboard

ORDERS orders@realvegancheese.org Real Vegan Cheese Marc will create/update an instruction for how to order, put stuff into inventory, make sure it gets paid for, etc.

Need to make sure that if we run out of DNA, we let somebody well-trained do the last transformation. Save some of the remainder E. coli when doing plasmid prep. (in broth in fridge) freezer. Need to be more careful about handling & storage of all our reagents USE QUARTZY FOR INVENTORY! add sublocations; include plasmid prep results; amount of reagents remaining etc.

Documentation cleanup Let's all pick a page on the wiki and help reorganize & clean it up ELSI pages, lab notebook, pages needed for iGEM (by Oct 17!) realvegancheese.org needs more content too (& more links to wiki)