Raw Experimental Notes June 2016

6/13/2016 - Maggie, Kristen, Patrik

Objective:

Found previous prepared plates (large & labeled RVC) Check for contamination: 4 -URA + Glucose (colorless), 5 (mixture of YPD + YPD + FOA):> decided not to use these and to repoured Pouring medias: YPD, SD (-Ura -Glu), 5-FOA; decided to pour whole pack (25), so 8 plates of each Volume of plate: 700 ml per plate; decide to make 20 ml of working media (so 20 x 8 plates = 160 ml) YPD = 50 g/L; SD- = 7.47 g/L; (need to add 20 g/L of glucose); 5-FOA: 0.5% see prep calculations here: https://docs.google.com/spreadsheets/d/1SRrFP4an20c3uaTYZRlUEeXM6md2O3ES7In1e4Jjqnk/edit#gid=0

Last night Patrik started some cultures Autoclave settings: 121 C (setting at 21)

Practices: Plates should be stored upside to avoid collecting contamination on lids) Contaminated plate submerged in bleachwater Preparing bleach: 1:10 bleach to tapwater (grab from restroom; not hand-washing water)