Western Blot of bovine casein

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May 2, 2015: Western Blot of Bovine Casein

Location: BioCurious

Participants: Priyanka, Johan, Advait, Maria, Patrik

Notes: Priyanka, Johan, Advait

Aims: Confirm presence of bovine casein protein

Material: for SDS page: SDS (10%) catalog number: SDS10-100 , distilled water For western blot: Gel loading tips (BioRad) Tris-Glycine SDS Running Buffer 10X stock (Novex Life Tech, Catalog number: LC2675) Tris-Glycine Mini Gels 4-20% 1.0mm x 10 wells/gel, (Catalog number: EC6025Box, Lot 14082110, received September 2014, possibly stored at -20C for a period of time) Protein ladder (10-250 kDa) (NEB, Catalog number: p7703S) 6X loading dye + SDS solution (Fermentas) Micellular casein powder from CCL stock (for positive control)


Results:

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