Difference between revisions of "2017 experiments"
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Cheryl Ching (talk | contribs) (→1/23/2017: edited 1/23 experiments section, added media recipes) |
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==1/23/2017== | ==1/23/2017== | ||
We autoclaved three different agar media and poured plates: | We autoclaved three different agar media and poured plates: | ||
# Nutrient Agar: | # Nutrient Agar: | ||
# | 2 g Carolina Nutrient Broth Mix | ||
# | 2.5 g Agar | ||
250 ml distilled water | |||
#CCL1: | |||
6.3 g soy powder | |||
0.63 g K2 HPO4 | |||
1.56 g NaOAc | |||
0.055 g magnesium chloride | |||
0.016 manganese chloride | |||
2.5 g agar | |||
6.25 glucose | |||
270 ml distilled water | |||
#MSY | |||
2% soy powder | |||
0.5% yeast extract | |||
1.5% agar | |||
We also inoculated [[bacterial cultures]] on 2% skim milk (Berkeley Farms DairyPure), from freezedried commercial cultures: | We also inoculated [[bacterial cultures]] on 2% skim milk (Berkeley Farms DairyPure), from freezedried commercial cultures: | ||
Revision as of 01:45, 1 February 2017
1/23/2017
We autoclaved three different agar media and poured plates:
- Nutrient Agar:
2 g Carolina Nutrient Broth Mix 2.5 g Agar 250 ml distilled water
- CCL1:
6.3 g soy powder 0.63 g K2 HPO4 1.56 g NaOAc 0.055 g magnesium chloride 0.016 manganese chloride 2.5 g agar 6.25 glucose 270 ml distilled water
- MSY
2% soy powder 0.5% yeast extract 1.5% agar
We also inoculated bacterial cultures on 2% skim milk (Berkeley Farms DairyPure), from freezedried commercial cultures:
- Streptococcus thermophilus
- Streptococcus salivarius ssp. thermophilus
- Lactobacillus paracasei