Difference between revisions of "Quantified IDT DNA (human kappa casein kex-) sample on nanodrop spectrophotometer to verify that the resuspension back in September worked. Checked for colony growth in 03Nov2014 streak plates."

From Real Vegan Cheese
Jump to navigation Jump to search
(Added experiment details)
 
 
(One intermediate revision by the same user not shown)
Line 1: Line 1:
'''November 03, 2014: Experiment to quantify the amount of DNA in the sample received from IDT'''
'''November 06, 2014: Experiment to quantify the amount of DNA in the sample received from IDT'''
*'''Location:''' BioCurious
*'''Location:''' BioCurious


Line 9: Line 9:


*'''Materials:'''
*'''Materials:'''
**''Nanodrop Sequencer''
**Nanodrop Sequencer''
**''1 ul of human kappa casein kex- DNA received from IDT that was resuspended in 10ul of TE/H20 back in Sept. 2014''
**1 ul of human kappa casein kex- DNA received from IDT that was resuspended in 10ul of TE/H20 back in Sept. 2014
**''1 ul of TE/H20 mixed in 1:1 ratio to duplicate resuspension buffer''
**1 ul of TE/H20 mixed in 1:1 ratio to duplicate resuspension buffer


*'''Methods:'''
*'''Methods:'''
**''1 ul of 50% TE was used to blank the nanodrop. Following this 1ul of the human kappa casein kex- DNA was used to verify the amount of DNA present.''
**1 ul of 50% TE was used to blank the nanodrop. Following this 1ul of the human kappa casein kex- DNA was used to verify the amount of DNA present.


*'''Results:'''
*'''Results:'''
The sample was found have a concentration of 17ng/ul of DNA.
The sample was found have a concentration of 17ng/ul of DNA.

Latest revision as of 20:38, 12 November 2014

November 06, 2014: Experiment to quantify the amount of DNA in the sample received from IDT

  • Location: BioCurious
  • Participants: Aaron Bryan, Johan
  • Notes: Johan
  • Aims: Before ordering the new electra kit, we wish to verify that we correctly suspending the IDT DNA.
  • Materials:
    • Nanodrop Sequencer
    • 1 ul of human kappa casein kex- DNA received from IDT that was resuspended in 10ul of TE/H20 back in Sept. 2014
    • 1 ul of TE/H20 mixed in 1:1 ratio to duplicate resuspension buffer
  • Methods:
    • 1 ul of 50% TE was used to blank the nanodrop. Following this 1ul of the human kappa casein kex- DNA was used to verify the amount of DNA present.
  • Results:

The sample was found have a concentration of 17ng/ul of DNA.

Retrieved from "http://wiki.realvegancheese.org/w/index.php?title=Quantified_IDT_DNA_(human_kappa_casein_kex-)_sample_on_nanodrop_spectrophotometer_to_verify_that_the_resuspension_back_in_September_worked._Checked_for_colony_growth_in_03Nov2014_streak_plates.&oldid=924"