Difference between revisions of "Raw Experimental Notes June 2016"
Jump to navigation
Jump to search
(Created page with "6/13/2016 - Maggie, Kristen, Patrik Objective: Found previous prepared plates (large & labeled RVC) Check for contamination: 4 -URA + Glucose (colorless), 5 (mixture of Y...") |
|||
| Line 5: | Line 5: | ||
Found previous prepared plates (large & labeled RVC) | * Found previous prepared plates (large & labeled RVC) | ||
Check for contamination: 4 -URA + Glucose (colorless), 5 (mixture of YPD + YPD + FOA):> decided not to use these and to | * Check for contamination: 4 -URA + Glucose (colorless), 5 (mixture of YPD + YPD + FOA):> decided not to use these and to repaired | ||
Pouring medias: YPD, SD (-Ura -Glu), 5-FOA; decided to pour whole pack (25), so 8 plates of each | * Pouring medias: YPD, SD (-Ura -Glu), 5-FOA; decided to pour whole pack (25), so 8 plates of each | ||
Volume of plate: 700 ml per plate; decide to make 20 ml of working media (so 20 x 8 plates = 160 ml) | * Volume of plate: 700 ml per plate; decide to make 20 ml of working media (so 20 x 8 plates = 160 ml) | ||
YPD = 50 g/L; SD- = 7.47 g/L; (need to add 20 g/L of glucose); 5-FOA: 0.5% see prep calculations here: https://docs.google.com/spreadsheets/d/1SRrFP4an20c3uaTYZRlUEeXM6md2O3ES7In1e4Jjqnk/edit#gid=0 | * YPD = 50 g/L; SD- = 7.47 g/L; (need to add 20 g/L of glucose); 5-FOA: 0.5% see prep calculations here: https://docs.google.com/spreadsheets/d/1SRrFP4an20c3uaTYZRlUEeXM6md2O3ES7In1e4Jjqnk/edit#gid=0 | ||
Last night Patrik started some cultures | * Last night Patrik started some cultures | ||
Autoclave settings: 121 C (setting at 21) | * Autoclave settings: 121 C (setting at 21) | ||
Practices: | Practices: | ||
Plates should be stored upside to avoid collecting contamination on lids) | * Plates should be stored upside to avoid collecting contamination on lids) | ||
Contaminated plate submerged in | * Contaminated plate submerged in bleach water | ||
Preparing bleach: 1:10 bleach to tapwater (grab from restroom; not hand-washing water) | * Preparing bleach: 1:10 bleach to tapwater (grab from restroom; not hand-washing water) | ||
Revision as of 03:53, 14 June 2016
6/13/2016 - Maggie, Kristen, Patrik
Objective:
- Found previous prepared plates (large & labeled RVC)
- Check for contamination: 4 -URA + Glucose (colorless), 5 (mixture of YPD + YPD + FOA):> decided not to use these and to repaired
- Pouring medias: YPD, SD (-Ura -Glu), 5-FOA; decided to pour whole pack (25), so 8 plates of each
- Volume of plate: 700 ml per plate; decide to make 20 ml of working media (so 20 x 8 plates = 160 ml)
- YPD = 50 g/L; SD- = 7.47 g/L; (need to add 20 g/L of glucose); 5-FOA: 0.5% see prep calculations here: https://docs.google.com/spreadsheets/d/1SRrFP4an20c3uaTYZRlUEeXM6md2O3ES7In1e4Jjqnk/edit#gid=0
- Last night Patrik started some cultures
- Autoclave settings: 121 C (setting at 21)
Practices:
- Plates should be stored upside to avoid collecting contamination on lids)
- Contaminated plate submerged in bleach water
- Preparing bleach: 1:10 bleach to tapwater (grab from restroom; not hand-washing water)