Difference between revisions of "SDS Page-Gel for all the bovine proteins"
Emi Nikolov (talk | contribs) |
Emi Nikolov (talk | contribs) |
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Method: | Method: | ||
Get 3 colonies from each plates in 1 centrifuge tube + resuspend into 20ml distilled water | Get 3 colonies from each plates in 1 centrifuge tube + resuspend into 20ml distilled water | ||
For casein touch 1 20-2w microliter pipette tip and rinse with 20 microliter dionised water in 1 casein control tube | For casein touch 1 20-2w microliter pipette tip and rinse with 20 microliter dionised water in 1 casein control tube | ||
Line 36: | Line 37: | ||
To run the gel | To run the gel | ||
Take 20 microliter of sample and 4 microliter of loading dye | Take 20 microliter of sample and 4 microliter of loading dye | ||
Boil all the samples for 10 minutes | Boil all the samples for 10 minutes | ||
Total amount of loading (1x) buffer we required 400 ml (10 ml of 10x L. Buffer into 90 ml distilled water) | Total amount of loading (1x) buffer we required 400 ml (10 ml of 10x L. Buffer into 90 ml distilled water) | ||
Run the gel on 150v for almost 1 hour | |||
Staining and destaining: Coomasie stain solution, destain solution kimwipes (or something else to absorb the coomassie) | |||
Result: | Result: We didn't get result on gel |
Revision as of 21:41, 7 December 2014
SDS Page-Gel for all of the bovine proteins
Monday Nov 24th
SDS Page-Gel for all of the bovine proteins
Participants: Wesley, Meenakshi, Nikola, Johan
Materials:
Casein
3B(100 fold diluted)
6B (100 fold diluted)
7 (100 fold diluted)
2B (100 fold diluted)
control 5FOA
transformed negative control
liquid culture
Bovine Beta B (8) - 20ml + 4ml loading dye
Bovine Alpha A (8) - 20ml + 4ml loading dye
Method:
Get 3 colonies from each plates in 1 centrifuge tube + resuspend into 20ml distilled water For casein touch 1 20-2w microliter pipette tip and rinse with 20 microliter dionised water in 1 casein control tube One sample take 1 colony from SFOA (as a negative control) One sample from control yeast transform plate
To run the gel
Take 20 microliter of sample and 4 microliter of loading dye Boil all the samples for 10 minutes
Total amount of loading (1x) buffer we required 400 ml (10 ml of 10x L. Buffer into 90 ml distilled water)
Run the gel on 150v for almost 1 hour
Staining and destaining: Coomasie stain solution, destain solution kimwipes (or something else to absorb the coomassie)
Result: We didn't get result on gel