Difference between revisions of "Electra cloning of"
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Emi Nikolov (talk | contribs) (Created page with "*'''Location:''' BioCurious *'''Participants:''' Johan, Wesley, Lafia and Nikola Name of the construct: P. FAKS: human kappa (kex-) S in PD1214 {| {{table}} | align="center...") |
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Revision as of 19:06, 16 December 2014
- Location: BioCurious
- Participants: Johan, Wesley, Lafia and Nikola
Name of the construct: P. FAKS: human kappa (kex-) S in PD1214
| ' | Component | ' | Tv = 20 ul |
| 1 | Distilled water | → | 1ul |
| 2 | Electra buffer (1x) | → | 2ul |
| 3 | Daughter vector (casein insert) | → | 1ul |
| 4 | Mother Vector (PD 1214) | → | 1ul |
| 5 | Electra enzyme mix | → | 15ul |
Combine the components
Incubate at 15-20 minutes in room temperature
Negative control plate, just competent cells (DH5alpha)
Positive control plate (PGLO)
5ul of plasmid plus 100ul of competent cells
Total Volume, 105ul - plating to plates