IGEM team meeting nodes 8/18/14

#Agenda 8/18/2014

Present: Stan, Ashley, Rebecca, Rachel, Carl, Craig, Patrik, Advait, Drew

\url{https://wiki.realvegancheese.org/index.php/Protease\_problems}
There are Kex2 protease sites inside our proteins; change a few amino acids to avoid them
Order *both* versions; native + Kex2 mutated
Fam20C is 2030 nucleotides; can't order gBlocks >2000bp; will cleave off 

RVC Board of directors: Marc, Craig, Ashley, Rachel, Ryan, Advait
Checking account is set up! 

UCSC update from Carl \& Advait:
according to Kim de Mora from iGEM, Advait did a great job!
Advait contacted iGEM HQ, and we are featured on iGEM's community page.

UCSC Meeting:
    Looked at UC poster symposium, had lunch, and then went into presentations. A scientist form NASA presented the keynote address, and then teams presented their projects.
    I presented our project by myself, and the audience really liked it! :) Then after that we had a few presentations from researchers on ethical issues and a panel on ethical issues. After that, we had a networking session, pizza, and bonfire on the beach.

Alternative Proteins, Alternative Futures: Panel \& Networking
\url{https://www.eventbrite.com/e/alternative-proteins-alternative-futures-panel-networking-tickets-12573271995}

LiAc still needs to be ordered - asap
Craig likes Quartzy as ordering system - ask Ashley for access

SDS-PAGE reagents need to be ordered (LifeTech)

#UCSC Speech:
##Powerpoint Presentation:  

   * 
Advait:
We, the Real Vegan Cheese team have emerged from the San Francisco Bay Area to create the world’s first *real * vegan cheese. 
 
Advait:
Our team is a collaborative effort between Oakland-based Counter Culture Labs and South Bay–centered BioCurious. 
 
Advait:
Our team consists of newbies and experts; from programmers, chemists, medical immunologists,  and bioinformaticians, to students and even a high schooler.
 
Advait:
We are attempting to make the world’s first  real vegan cheese by engineering normal baker's yeast (S. cerevisiae) to express milk protein (casein), purifying the protein, creating a milk-substitute by blending in vegan replacements for lactose and milk fat, and finally turning the resulting milk-substitute into semi-hard cheese like gouda using the regular cheese-making process.
 
Advait:
We are also currently running an indiegogo campaign (explain if they don’t know what it is), and we have collected over $24,000 dollars for our cause. We have been featured in over 86 blog posts and articles. 
 
Advait:
Our motivations/missions include the following:
(read off the powerpoint)
 
Advait:
We have faced numerous challenges along the way, which include: 
                -coordinating the effort of over 30 team members
                -creating and running a successful indiegogo campaign
                -Explaining the actual process (how we will manage to make cheese from an animal DNA 
sequence) to the general public
-Confronting the fears of the mis-informed (there will be no GMO material left in the final
cheese) and explaining to them how this will not cause problems
 
Advait:
                This is our first milestone. Our other milestones can be found at \url{https://pad.riseup.net/p/vegancheesemilestones}
 
Advait:
                We have been featured in over 86 blog posts and articles. You can find more information on the following websites: (read off of powerpoint). Thanks for watching!!!