IGEM team meeting notes 7/20/14

Agenda: 07/20/14

Call-in info: https://zoom.us/j/820128495 Or, go to https://zoom.us/join and enter meeting ID: 820 128 495

Note: Alisa Cordesius from IndieGogo may give us a call with suggestions for how to improve our campaign!

I. Intro/Go around Meeting Attendees: (zoom): Advait , Maria, Wes CCL: Patrik, Lou, Beth, Rachel, Ryan, Jamie, Ahnon

II. Report back to-does from last meeting TO-DO list from last week:

1. Yeast Strain - finish registration on ATCC (Credit refs) 2. Next meeting at BioCurious: have a planned experiment ready for the meeting; contact Nicola to coordinate details 3. Contact sequentech about sequencing reactions and primer design - 4. Modify experiment timeline google doc: add additional sheets to overall document, one for each month, to break monthly calendar schedule into a week-by-week schedule for precise tuning of progress

III. IndieGogo progress Urgent: Anybody for a live Radio Interview tomorrow morning? https://groups.google.com/forum/#!topic/ccl-igem/h0b2vIT6Riw Jamie will do the interview Help optimize our content! Lots of good suggestions from Antony & others - need more people to help implement. What is our *next* stretch goal. Should have a graphic showing our funding horizon doesn't end at $20K Next update: what? Could do 3D printed prototypes Contact remaining "Tell Us Why We're Wrong" and "Ask a Biohacker" donors. (see https://pad.riseup.net/p/socialcheesemedia ) Write new article to submit to other magazines/newspapers/news stations or get more people to write articles for us https://docs.google.com/document/d/1UsZk7yiUDEB118iMZ22Fg3j2nRMyBxvLEmku_bfl25k/edit

IIIa Stretch goals Mammoth Cheese - problem - unecc glamourous, not taken seriously Bioreactor - 1 large - need might not be understood by general public, but sounds cool Bioreactor - several small Micelle equipment: particle analyzer Endangered - black-footed ferret - too glam but may raise awareness of endangered species... but might be too much new info which muddies/decreases understanding of both projects

Media Contacts Wired - we have several contacts KQED - Patrik talked to her

Why cheese instead of Milk? Cheese is harder (micelle formation, etc.) Milk is being done by Muufri

IV. Complete the new safety form for igem together

   http://igem.org/Safety/Safety_Form?team_id=1531

- Who has taken the Biocurious Safety Training and who hasn't? Has: Has not: Or, complete the safety training together (DO NOT USE QUIZ LINK YET!)

V. Work on the experiment plan/schedule

-https://docs.google.com/spreadsheets/d/1NOPblWr9_pIHMst0Nvy__7J_n97LNwEsz2ujtZ3yFBo/edit#gid=0 -https://www.google.com/calendar/render?pli=1

Molecular Biology Experiments

Run SDS-PAGE gels on K-casein from E. coli Order primers to verify construct Send out K-casein for sequencing Make competent yeast Transform yeast Run SDS-PAGE gels on K-casein from yeast

Things we need:

   - Uracil
   - Reagents to induce competence
   - Buy gels or pour our own? BUY! We gots $
   - SDSbuffer, loading dye and Molecular Weight marker.
   - Primers
   - 

Cheese experiment 2

Tenatively scheduled for the Omni 4799 Shattuck Ave July 28 7PM

Can we dissolve casein powder, and make cheese out of it?

Stuff to bring:

• Matts high-shear blender
• jewelry cleaning sonicator (patrik will bring)
• Do we have lab-sonicators? (may be more adjustable)
• heater (juul will verify if omni has stoves)
• pH meter (juul will order one, lou will check home depot and aquariam store)
• chymosin (juul will bring)
• white vinegar (lou will bring)
• thermometer (digital ?) (juul & lou will bring)
• cheese cloth (Patrik will bring)
• pot (lou will bring)
• CaCl (juul will bring)
• test tubes / glasswares
• ice (lou will bring)

David Poole agreed to bring the following. 1- micellar casein powder (oddly missing from our list) 2- whole milk (control) 3- fat free milk 4- milk-free fat 5- citric acid 6- extra calcium salt 7- extra rennin/chymosin 8- extra jewelry sonicator 9- papers on prior work from the journal of dairy science 10- additional stuff I've found useful for making cheese (e.g. a salad spinner, thermometer etc)

Parameters to vary: pH, Ca, T, sonication/shear test using chymosin, and settling over time

ToDo:

• Collect all papers relevant to experiment, and give access to everyone (juul)
• Solicit questions for ask a biohacker and tell us why we're wrong (juul)
• Research protein purification methods (juul)
• Organize / set up inventory